Tracking the cargo of extracellular symbionts into host tissues with correlated electron microscopy and nanoscale secondary ion mass spectrometry imaging.

Détails

ID Serval
serval:BIB_F881AD484791
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Tracking the cargo of extracellular symbionts into host tissues with correlated electron microscopy and nanoscale secondary ion mass spectrometry imaging.
Périodique
Cellular microbiology
Auteur⸱e⸱s
Cohen S.K., Aschtgen M.S., Lynch J.B., Koehler S., Chen F., Escrig S., Daraspe J., Ruby E.G., Meibom A., McFall-Ngai M.
ISSN
1462-5822 (Electronic)
ISSN-L
1462-5814
Statut éditorial
Publié
Date de publication
04/2020
Peer-reviewed
Oui
Volume
22
Numéro
4
Pages
e13177
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Extracellular bacterial symbionts communicate biochemically with their hosts to establish niches that foster the partnership. Using quantitative ion microprobe isotopic imaging (nanoscale secondary ion mass spectrometry [NanoSIMS]), we surveyed localization of <sup>15</sup> N-labelled molecules produced by the bacterium Vibrio fischeri within the cells of the symbiotic organ of its host, the Hawaiian bobtail squid, and compared that with either labelled non-specific species or amino acids. In all cases, two areas of the organ's epithelia were significantly more <sup>15</sup> N enriched: (a) surface ciliated cells, where environmental symbionts are recruited, and (b) the organ's crypts, where the symbiont population resides in the host. Label enrichment in all cases was strongest inside host cell nuclei, preferentially in the euchromatin regions and the nucleoli. This permissiveness demonstrated that uptake of biomolecules is a general mechanism of the epithelia, but the specific responses to V. fischeri cells recruited to the organ's surface are due to some property exclusive to this species. Similarly, in the organ's deeper crypts, the host responds to common bacterial products that only the specific symbiont can present in that location. The application of NanoSIMS allows the discovery of such distinct modes of downstream signalling dependent on location within the host and provides a unique opportunity to study the microbiogeographical patterns of symbiotic dialogue.
Mots-clé
15N-labeled bacteria, host-microbe communication, squid-vibrio
Pubmed
Création de la notice
01/04/2020 20:33
Dernière modification de la notice
15/07/2020 5:26
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