Glial hyaluronate-binding protein expression in aggregating brain cell cultures.

Détails

ID Serval
serval:BIB_F66F9E05AA18
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Glial hyaluronate-binding protein expression in aggregating brain cell cultures.
Périodique
Developmental Neuroscience
Auteur(s)
Monnet-Tschudi F., Zurich M.G., Asher R., Honegger P.
ISSN
0378-5866[print], 0378-5866[linking]
Statut éditorial
Publié
Date de publication
1993
Volume
15
Numéro
6
Pages
395-402
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
We analyzed the expression of glial hyaluronate-binding protein (GHAP), an integral component of the extracellular matrix, in aggregating brain cell cultures of fetal rat telencephalon using immunofluorescence. GHAP immunoreactivity appeared after 1 week in culture, simultaneous with the first deposits of myelin basic protein, and showed a development-dependent increase. Comparison of glia-enriched and neuron-enriched cultures showed that only glial cells express GHAP. Three peptide growth factors, epidermal growth factor, fibroblast growth factor and platelet-derived growth factor, which are known to stimulate the differentiation of glial cells, modulated the deposit of GHAP immunoreactivity. The 3-dimensional structure of aggregate cultures promoted GHAP deposition, suggesting that cell-cell interactions are required for extracellular matrix formation. Furthermore GHAP production seemed to depend on the developmental stage of the glial cells.
Mots-clé
Animals, Blotting, Western, Brain/cytology, Brain Chemistry/physiology, Carrier Proteins/biosynthesis, Cell Aggregation/physiology, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Epidermal Growth Factor/metabolism, Extracellular Matrix/metabolism, Fibroblast Growth Factors/metabolism, Fluorescent Antibody Technique, Glial Fibrillary Acidic Protein/metabolism, Immunohistochemistry, Nerve Tissue Proteins/biosynthesis, Platelet-Derived Growth Factor/metabolism, Rats, Rats, Wistar, Versicans
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 17:22
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