Macrophage migration inhibitory factor (MIF) in meningococcal septic shock and experimental human endotoxemia

Détails

ID Serval
serval:BIB_F61BD0D1A6DE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Macrophage migration inhibitory factor (MIF) in meningococcal septic shock and experimental human endotoxemia
Périodique
Shock
Auteur(s)
Sprong  T., Pickkers  P., Geurts-Moespot  A., van der Ven-Jongekrijg  J., Neeleman  C., Knaup  M., Leroy  D., Calandra  T., van der Meer  J. W., Sweep  F., van Deuren  M.
ISSN
1073-2322 (Print)
Statut éditorial
Publié
Date de publication
05/2007
Volume
27
Numéro
5
Pages
482-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Macrophage migration inhibitory factor (MIF) is a mediator of innate immunity and important in the pathogenesis of septic shock. Lipopolysaccharide (LPS) and tumor necrosis factor (TNF) alpha are reported to be inducers of MIF. We studied MIF and cytokines in vivo in patients with meningococcal disease, in human experimental endotoxemia, and in whole blood cultures using a newly developed sensitive and specific enzyme-linked immunosorbent assay. Twenty patients with meningococcal disease were investigated. For the human endotoxemia model, 8 healthy volunteers were intravenously injected with 2 ng/kg Escherichia coli LPS. Whole blood from healthy volunteers was incubated with LPS or heat-killed meningococci. Macrophage migration inhibitory factor concentration in blood was increased during meningococcal disease and highest in the patients presenting with shock compared with patients without shock. Plasma concentration of MIF correlated with disease severity, the presence of shock and with the cytokines interleukin (IL) 1beta, IL-10, IL-12, and vascular endothelial growth factor, but not with TNF-alpha. MIF was not detected in blood in experimental endotoxemia, nor after stimulation of whole blood with LPS or meningococci, although high levels of TNF-alpha were seen in both models. In conclusion, MIF is increased in patients with meningococcal disease and highest in the presence of shock. Macrophage migration inhibitory factor cannot be detected in a human endotoxemia model and is not produced by whole blood cells incubated with LPS or meningococci.
Mots-clé
Adolescent Adult Child Child, Preschool Cytokines/blood Endotoxemia/*blood/chemically induced/pathology Enzyme-Linked Immunosorbent Assay Female Humans Immunity, Natural/drug effects Interleukin-10/blood Interleukin-12/blood Interleukin-1beta/blood Lipopolysaccharides/administration & dosage Macrophage Migration-Inhibitory Factors/*blood Male Meningococcal Infections/*blood/microbiology/pathology Neisseria meningitidis/growth & development Shock, Septic/*blood/pathology Time Factors Tumor Necrosis Factor-alpha/blood Vascular Endothelial Growth Factor A/blood
Pubmed
Web of science
Création de la notice
25/01/2008 14:28
Dernière modification de la notice
20/08/2019 17:22
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