Class I MHC molecules on hematopoietic cells can support intrathymic positive selection of T cell receptor transgenic T cells.

Détails

ID Serval
serval:BIB_F60D2BE4EC75
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Class I MHC molecules on hematopoietic cells can support intrathymic positive selection of T cell receptor transgenic T cells.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Zerrahn J., Volkmann A., Coles M.C., Held W., Lemonnier F.A., Raulet D.H.
ISSN
0027-8424
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
96
Numéro
20
Pages
11470-11475
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The identity of cells that mediate positive selection of CD8(+) T cells was investigated in two T cell receptor (TCR) transgenic systems. Irradiated beta(2)-microglobulin mutant mice or mice with mutations in both the K(b) and D(b) genes were repopulated with fetal liver cells from class I(+) TCR transgenic mice. In the case of the 2C TCR, mature transgene-expressing CD8(+) T cells appeared in the thymuses of the chimeras and in larger numbers in the peripheral lymphoid organs. These CD8(+) T cells were functional, exhibited a naive, resting phenotype, and were mostly thymus-dependent. Their development depended on donor cell class I expression. These results establish that thymic hematopoietic cells can direct positive selection of CD8(+) T cells expressing a conventional TCR. In contrast, no significant development of HY (male antigen)-TCR(+) CD8(+) T cells was observed in class I(+) into class I-deficient chimeras. These data suggest that successful positive selection directed by hematopoietic cells depends on specific properties of the TCR or its thymic ligands. The possibility that hematopoietic cell-induced, positive selection occurs only with TCRs that exhibit relatively high avidity interactions with selecting ligands in the thymus is discussed.
Mots-clé
Animals, CD8-Positive T-Lymphocytes/physiology, Chimera, Hematopoietic Stem Cells/physiology, Histocompatibility Antigens Class I/physiology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Receptors, Antigen, T-Cell/physiology, Thymus Gland/cytology
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/01/2008 15:24
Dernière modification de la notice
20/08/2019 16:22
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