CD8+ cytotoxic T lymphocyte activation by soluble major histocompatibility complex-peptide dimers.

Détails

ID Serval
serval:BIB_F605EBF0C6BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
CD8+ cytotoxic T lymphocyte activation by soluble major histocompatibility complex-peptide dimers.
Périodique
The Journal of biological chemistry
Auteur(s)
Cebecauer M., Guillaume P., Mark S., Michielin O., Boucheron N., Bezard M., Meyer B.H., Segura J.M., Vogel H., Luescher I.F.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
280
Numéro
25
Pages
23820-23828
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
CD8+ cytotoxic T lymphocyte (CTL) can recognize and kill target cells that express only a few cognate major histocompatibility complex class I-peptide (pMHC) complexes. To better understand the molecular basis of this sensitive recognition process, we studied dimeric pMHC complexes containing linkers of different lengths. Although dimers containing short (10-30-A) linkers efficiently bound to and triggered intracellular calcium mobilization and phosphorylation in cloned CTL, dimers containing long linkers (> or = 80 A) did not. Based on this and on fluorescence resonance energy transfer experiments, we describe a dimeric binding mode in which two T cell receptors engage in an anti-parallel fashion two pMHC complexes facing each other with their constant domains. This binding mode allows integration of diverse low affinity interactions, which increases the overall binding and, hence, the sensitivity of antigen recognition. In proof of this, we demonstrated that pMHC dimers containing one agonist and one null ligand efficiently activate CTL, corroborating the importance of endogenous pMHC complexes in antigen recognition.
Mots-clé
Amino Acid Sequence, Blotting, Western, CD8-Positive T-Lymphocytes/immunology, Cell Line, Dimerization, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Humans, Immunoprecipitation, Lymphocyte Activation, Major Histocompatibility Complex, Models, Molecular, Peptides/chemistry
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:20
Dernière modification de la notice
20/08/2019 17:22
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