The fetal hypothalamus has the potential to generate cells with a gonadotropin releasing hormone (GnRH) phenotype.

Détails

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Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_F5FE9EED6C4B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The fetal hypothalamus has the potential to generate cells with a gonadotropin releasing hormone (GnRH) phenotype.
Périodique
PloS one
Auteur⸱e⸱s
Salvi R., Arsenijevic Y., Giacomini M., Rey J.P., Voirol M.J., Gaillard R.C., Risold P.Y., Pralong F.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
4
Numéro
2
Pages
e4392
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Neurospheres (NS) are colonies of neural stem and precursor cells capable of differentiating into the central nervous system (CNS) cell lineages upon appropriate culture conditions: neurons, and glial cells. NS were originally derived from the embryonic and adult mouse striatum subventricular zone. More recently, experimental evidence substantiated the isolation of NS from almost any region of the CNS, including the hypothalamus.
Here we report a protocol that enables to generate large quantities of NS from both fetal and adult rat hypothalami. We found that either FGF-2 or EGF were capable of inducing NS formation from fetal hypothalamic cultures, but that only FGF-2 is effective in the adult cultures. The hypothalamic-derived NS are capable of differentiating into neurons and glial cells and most notably, as demonstrated by immunocytochemical detection with a specific anti-GnRH antibody, the fetal cultures contain cells that exhibit a GnRH phenotype upon differentiation.
This in vitro model should be useful to study the molecular mechanisms involved in GnRH neuronal differentiation.

Mots-clé
Animals, Astrocytes/cytology, Biomarkers/metabolism, Cell Differentiation, Cell Proliferation, Cell Shape, Fetus/cytology, Gonadotropin-Releasing Hormone/metabolism, Hypothalamus/cytology, Hypothalamus/embryology, Neuroepithelial Cells/cytology, Neurons/cytology, Oligodendroglia/cytology, Organ Specificity, Phenotype, Rats, Rats, Wistar, Stem Cells/cytology, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/04/2009 18:33
Dernière modification de la notice
20/08/2019 16:22
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