Improved diagnoses of autoimmune hepatitis using an anti-actin ELISA.

Détails

ID Serval
serval:BIB_F5F52B19AB09
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Improved diagnoses of autoimmune hepatitis using an anti-actin ELISA.
Périodique
Journal of Clinical Laboratory Analysis
Auteur⸱e⸱s
Aubert V., Pisler I.G., Spertini F.
ISSN
1098-2825 (Electronic)
ISSN-L
0887-8013
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
22
Numéro
5
Pages
340-345
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Résumé
The presence of antismooth muscle antibodies is one of the diagnostic criteria of autoimmune hepatitis. We evaluated a new anti-F-actin ELISA test and compared it with indirect immunofluorescence assay (IIFA) for antismooth muscle antibodies (ASMA). Two hundred and nine serum samples (35 autoimmune hepatitis, 174 other hepatopathies and control sera) were tested by IIFA on mouse stomach kidney sections for ASMA and by the Quanta Lite Actin ELISA for anti-F-actin antibodies. ASMA were detected in 26 of 35 sera from autoimmune hepatitis (74%) as compared with 25 (71%) with anti-actin antibodies, as well as in 25 of 49 (51%) samples from viral hepatitis as compared with 7 (14%) with anti-actin antibodies. With regards to autoimmune hepatitis, though sensitivity (74.3 vs 71.4%) and negative predictive value (93.5 vs 93.9%) of ASMA and anti-actin ELISA were comparable, anti-actin ELISA was significantly better than ASMA IIFA in terms of specificity (89.7 vs 74.7%), and positive predictive value (58.1 vs 37.1%). Although frequently positive in HCV samples, a comparable sensitivity but better specificity makes the anti-actin ELISA a useful tool in combination with ASMA IIFA for the screening and diagnosis of autoimmune hepatitis.
Mots-clé
Actins/immunology, Animals, Autoantibodies/blood, Enzyme-Linked Immunosorbent Assay/methods, Fluorescent Antibody Technique, Indirect, Hepatitis, Autoimmune/blood, Hepatitis, Autoimmune/diagnosis, Humans, Mice, Predictive Value of Tests, Retrospective Studies
Pubmed
Web of science
Création de la notice
15/12/2008 17:51
Dernière modification de la notice
20/08/2019 16:22
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