Electrophysiological Characterization of Auditory Neuron Response Profiles on Multi-Electrode Arrays

Détails

Ressource 1Télécharger: Mémoire no 3535 Mme Brauer.pdf (9153.45 [Ko])
Etat: Public
Version: Après imprimatur
ID Serval
serval:BIB_F5C44B8C8A57
Type
Mémoire
Sous-type
(Mémoire de) maîtrise (master)
Collection
Publications
Institution
Titre
Electrophysiological Characterization of Auditory Neuron Response Profiles on Multi-Electrode Arrays
Auteur(s)
BRAUER S.-K.
Directeur(s)
SENN P.
Codirecteur(s)
ROCCIO M.
Détails de l'institution
Université de Lausanne, Faculté de biologie et médecine
Statut éditorial
Acceptée
Date de publication
2016
Langue
anglais
Nombre de pages
30
Résumé
Objective. Cochlear implants (CI) are a very successful type of neuroprosthetic device, which are globally used as gold standard treatment for deafness. Despite the many advances made in the development of cochlear implants, some limitations still remain, such as poor frequency resolution and high-energy consumption. It is thought that the anatomical gap between the implanted electrode array and the spiral ganglion neurons (SGNs) might be one of the limiting factors. Therefore we analyzed the electrophysiological features of SGNs growing in close contact with an electrode array.
Approach. We characterized SGN responses to extracellular stimulation in vitro using multi-electrode arrays (MEAs). SGN explants were obtained either from the cochlear’s apex or the base and were cultured with two different neurotrophic factors – brain-derived neurotrophic factor (BDNF) and/or neurotrophin-3 (NT- 3) – that are known to have opposing influences on apex and base.
Main results. SGN explant culturing was successful in all six conditions: independently from the anatomical location or the supplemented neurotrophins, spontaneous and stimulated neuronal activity could be recorded in all six conditions. A difference in neuronal threshold values as well as in their accommodation was observed between apex and base depending on the supplemented neurotrophins.
Significance. Understanding the relation of neurotrophic factors to apical and basal SGNs could help to improve the contact of SGNs with the electrode array as well as to include location-specific firing features of cochlear SGNs in the stimulation patterns.
Mots-clé
cochlea, auditory, serial ganglion, multi-electrode array, electrophysiology
Création de la notice
06/09/2017 10:53
Dernière modification de la notice
20/08/2019 17:22
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