Expression of mu-opiate receptor in human epidermis and keratinocytes

Détails

ID Serval
serval:BIB_F53E4A753D44
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression of mu-opiate receptor in human epidermis and keratinocytes
Périodique
Journal of Investigative Dermatology
Auteur⸱e⸱s
Bigliardi  P. L., Bigliardi-Qi  M., Buechner  S., Rufli  T.
ISSN
0022-202X (Print)
Statut éditorial
Publié
Date de publication
08/1998
Volume
111
Numéro
2
Pages
297-301
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
There is increasing evidence that neurotransmitters play a crucial role in skin physiology and pathology. The expression and production of proopiomelanocortin molecules such as beta-endorphin in human epidermis suggest that an opiate receptor is present in keratinocytes. In this paper we show that human epidermal keratinocytes express a mu-opiate receptor on both the mRNA level and the protein level. Performing polymerase chain reaction with cDNA libraries from human epidermal keratinocytes gave the polymerase chain reaction products of the expected length, which were confirmed as mu-opiate receptors by Southern blot analysis. Using in situ hybridization techniques with a specific probe for mu-opiate receptors we detected the receptor in human epidermis. There was a cytoplasmic expression in all layers of the epidermis, which was more distinct in the suprabasal layers. Immunohistochemistry using the mu-opiate receptor-specific antibody indicates that epidermis expresses protein as well, and that the protein level is more elevated in the basal layer. The correlation between the locations of both mRNA and protein expression in skin indicates that the mu-opiate receptor has not only been transcribed but also has a specific function. To prove a function of the receptor we performed a functional assay using skin organ cultures from human skin transplants. After 48 h incubation with Naloxone or beta-endorphin the expression of the mu-opiate receptor in epidermis was significantly downregulated compared with the control. These results show that a functional receptor indeed exists in human epidermis.
Mots-clé
Animals Epidermis/*chemistry Humans Keratinocytes/*chemistry Naloxone/pharmacology Organ Culture Techniques RNA, Messenger/analysis Rats Receptors, Opioid, mu/*analysis/genetics beta-Endorphin/pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:30
Dernière modification de la notice
20/08/2019 16:22
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