NanoBiT System and Hydrofurimazine for Optimized Detection of Viral Infection in Mice-A Novel in Vivo Imaging Platform.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_F4F6102BC551
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
NanoBiT System and Hydrofurimazine for Optimized Detection of Viral Infection in Mice-A Novel in Vivo Imaging Platform.
Périodique
International journal of molecular sciences
Auteur⸱e⸱s
Gaspar N., Zambito G., Dautzenberg IJC, Cramer S.J., Hoeben R.C., Lowik C., Walker J.R., Kirkland T.A., Smith T.P., van Weerden W.M., de Vrij J., Mezzanotte L.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Statut éditorial
Publié
Date de publication
15/08/2020
Peer-reviewed
Oui
Volume
21
Numéro
16
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Reporter genes are used to visualize intracellular biological phenomena, including viral infection. Here we demonstrate bioluminescent imaging of viral infection using the NanoBiT system in combination with intraperitoneal injection of a furimazine analogue, hydrofurimazine. This recently developed substrate has enhanced aqueous solubility allowing delivery of higher doses for in vivo imaging. The small high-affinity peptide tag (HiBiT), which is only 11 amino-acids in length, was engineered into a clinically used oncolytic adenovirus, and the complementary large protein (LgBiT) was constitutively expressed in tumor cells. Infection of the LgBiT expressing cells with the HiBiT oncolytic virus will reconstitute NanoLuc in the cytosol of the cell, providing strong bioluminescence upon treatment with substrate. This new bioluminescent system served as an early stage quantitative viral transduction reporter in vitro and also in vivo in mice, for longitudinal monitoring of oncolytic viral persistence in infected tumor cells. This platform provides novel opportunities for studying the biology of viruses in animal models.
Mots-clé
Adenoviridae/genetics, Animals, Cell Line, Tumor, HEK293 Cells, Humans, Injections, Intraperitoneal, Luminescent Agents/administration & dosage, Luminescent Agents/pharmacokinetics, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Oligopeptides/genetics, Oligopeptides/metabolism, Oncolytic Viruses/genetics, Optical Imaging/methods, Recombinant Proteins/genetics, Virus Diseases/diagnostic imaging, bioluminescence imaging, hibit tag, hydrofurimazine, nanobit system, oncolytic virus
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/09/2020 10:10
Dernière modification de la notice
30/04/2021 6:16
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