Chromosomal integration, tandem amplification, and deamplification in Pseudomonas putida F1 of a 105-kilobase genetic element containing the chlorocatechol degradative genes from Pseudomonas sp. Strain B13.

Détails

ID Serval
serval:BIB_F4740901DD86
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Chromosomal integration, tandem amplification, and deamplification in Pseudomonas putida F1 of a 105-kilobase genetic element containing the chlorocatechol degradative genes from Pseudomonas sp. Strain B13.
Périodique
Journal of Bacteriology
Auteur(s)
Ravatn R., Studer S., Springael D., Zehnder A.J., van der Meer J.R.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
180
Numéro
17
Pages
4360-4369
Langue
anglais
Résumé
Analysis of chlorobenzene-degrading transconjugants of Pseudomonas putida F1 which had acquired the genes for chlorocatechol degradation (clc) from Pseudomonas sp. strain B13 revealed that the clc gene cluster was present on a 105-kb amplifiable genetic element (named the clc element). In one such transconjugant, P. putida RR22, a total of seven or eight chromosomal copies of the entire genetic element were present when the strain was cultivated on chlorobenzene. Chromosomal integrations of the 105-kb clc element occurred in two different loci, and the target sites were located within the 3' end of glycine tRNA structural genes. Tandem amplification of the clc element was preferentially detected in one locus on the F1 chromosome. After prolonged growth on nonselective medium, transconjugant strain RR22 gradually diverged into subpopulations with lower copy numbers of the clc element. Two nonadjacent copies of the clc element in different loci always remained after deamplification, but strains with only two copies could no longer use chlorobenzene as a sole substrate. This result suggests that the presence of multiple copies of the clc gene cluster was a prerequisite for the growth of P. putida RR22 on chlorobenzene and that amplification of the element was positively selected for in the presence of chlorobenzene.
Mots-clé
Base Sequence, Biodegradation, Environmental, Chlorobenzoates/metabolism, Chromosomes, Bacterial, Conjugation, Genetic, Cosmids, Culture Media, Electrophoresis, Gel, Pulsed-Field, Gene Amplification, Molecular Sequence Data, Nucleic Acid Hybridization, Pseudomonas putida/genetics, Pseudomonas putida/growth & development, Restriction Mapping
Pubmed
Web of science
Création de la notice
21/01/2008 14:35
Dernière modification de la notice
20/08/2019 17:21
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