DNA-mediated transport of the intermediate filament protein vimentin into the nucleus of cultured cells.

Détails

ID Serval
serval:BIB_F42C3D5E14F3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
DNA-mediated transport of the intermediate filament protein vimentin into the nucleus of cultured cells.
Périodique
Journal of cell science
Auteur(s)
Hartig R., Shoeman R.L., Janetzko A., Tolstonog G., Traub P.
ISSN
0021-9533 (Print)
ISSN-L
0021-9533
Statut éditorial
Publié
Date de publication
18/12/1998
Peer-reviewed
Oui
Volume
111 ( Pt 24)
Pages
3573-3584
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
A number of characteristic properties of intermediate filament (IF) proteins, such as nucleic acid-binding activity, affinity for histones and structural relatedness to transcription factors and nuclear matrix proteins, in conjunction with the tight association of IFs with the nucleus, suggest that these proteins might also fulfill nuclear functions in addition to their structure-organizing and -stabilizing activities in the cytoplasm. Yet, cytoplasmic IF proteins do not possess nuclear localization signals. In a search for carriers capable of transporting the IF protein vimentin into the nucleus, complexes of FITC-vimentin with various DNAs were microinjected into the cytoplasm of cultured cells and the intracellular distribution of the protein was followed by confocal laser scanning microscopy. The single-stranded oligodeoxyribonucleotides oligo(dG)25, oligo[d(GT)12G] and oligo[d(G3T2A)4G] proved to be excellent nuclear carriers for vimentin. However, in fibroblasts, fluorescence-labeled vimentin taken up by the nuclei remained undetectable with affinity-purified, polyclonal anti-vimentin antibody, whereas it was readily identifiable in the nuclei of microinjected epithelial cells in this way. Moreover, when FITC-vimentin was preinjected into fibroblasts and allowed to assemble into the endogenous vimentin filament system, it was still transferred into the nucleus by post-injected oligo(dG)25, although to a lesser extent. Superhelical circular DNAs, like pBR322, SV40 and mitochondrial DNA, were also characterized by considerable capacities for nuclear vimentin transport; these transport potentials were totally destroyed by relaxation or linearization of the DNA molecules. Nevertheless, certain linear double-stranded DNA molecules with a high affinity for vimentin IFs, such as repetitive telomere and centromere or mobile long interspersed repeat (LINE) DNA, could carry FITC-vimentin into the nucleus. This was also true for a 375 bp extrachromosomal linear DNA fragment which occurs in the cytoplasm of mouse tumor cells and which is capable of immortalizing human lymphocytes. On the basis of these results, it appears very likely that cellular and viral products of reverse transcription as well as other extrachromosomal DNAs, which are circular, superhelical and apparently shuttling between the cytoplasm and the nucleus (eccDNA), are constantly loaded with vimentin in vimentin-positive cells. Since such DNAs are considered as markers of genomic instability, it is conceivable that vimentin directly participates as an architectural, chromatin-modifying protein in recombinatorial processes set off by these DNAs in the nucleus.
Mots-clé
Animals, Biological Transport, Cats, Cell Nucleus/metabolism, Cells, Cultured, Chickens, Cricetinae, Cytoplasm/metabolism, DNA/metabolism, Fluorescein-5-isothiocyanate/metabolism, Fluorescent Dyes/metabolism, Guinea Pigs, Humans, Intracellular Fluid/metabolism, Mice, Microinjections, Microscopy, Confocal, Oligodeoxyribonucleotides/metabolism, Protein Binding, Vimentin/metabolism
Pubmed
Web of science
Création de la notice
15/12/2017 17:28
Dernière modification de la notice
14/01/2020 7:26
Données d'usage