Phosphorylation by casein kinase 2 facilitates rRNA gene transcription by promoting dissociation of TIF-IA from elongating RNA polymerase I.

Détails

ID Serval
serval:BIB_F422FF61B727
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Phosphorylation by casein kinase 2 facilitates rRNA gene transcription by promoting dissociation of TIF-IA from elongating RNA polymerase I.
Périodique
Molecular and Cellular Biology
Auteur(s)
Bierhoff H., Dundr M., Michels A.A., Grummt I.
ISSN
1098-5549[electronic], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
28
Numéro
16
Pages
4988-4998
Langue
anglais
Résumé
The protein kinase casein kinase 2 (CK2) phosphorylates different components of the RNA polymerase I (Pol I) transcription machinery and exerts a positive effect on rRNA gene (rDNA) transcription. Here we show that CK2 phosphorylates the transcription initiation factor TIF-IA at serines 170 and 172 (Ser170/172), and this phosphorylation triggers the release of TIF-IA from Pol I after transcription initiation. Inhibition of Ser170/172 phosphorylation or covalent tethering of TIF-IA to the RPA43 subunit of Pol I inhibits rDNA transcription, leading to perturbation of nucleolar structure and cell cycle arrest. Fluorescence recovery after photobleaching and chromatin immunoprecipitation experiments demonstrate that dissociation of TIF-IA from Pol I is a prerequisite for proper transcription elongation. In support of phosphorylation of TIF-IA switching from the initiation into the elongation phase, dephosphorylation of Ser170/172 by FCP1 facilitates the reassociation of TIF-IA with Pol I, allowing a new round of rDNA transcription. The results reveal a mechanism by which the functional interplay between CK2 and FCP1 sustains multiple rounds of Pol I transcription.
Mots-clé
Amino Acid Sequence, Animals, Casein Kinase II/metabolism, Cell Cycle, Cell Nucleolus/metabolism, Cell Proliferation, DNA, Ribosomal/genetics, Humans, Mice, Models, Biological, Molecular Sequence Data, NIH 3T3 Cells, Phosphoprotein Phosphatases/metabolism, Phosphorylation, Phosphoserine/metabolism, Pol1 Transcription Initiation Complex Proteins, Promoter Regions, Genetic/genetics, Protein Binding, RNA Polymerase I/metabolism, RNA Precursors/biosynthesis, RNA, Ribosomal/genetics, Transcription Factors/chemistry, Transcription Factors/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/06/2009 12:32
Dernière modification de la notice
20/08/2019 17:21
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