A stain for ischaemic or excessively stimulated neurons.

Détails

ID Serval
serval:BIB_F3297C9A73D9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A stain for ischaemic or excessively stimulated neurons.
Périodique
Neuroscience
Auteur(s)
Clarke P.G.H., Nussbaumer J.C.
ISSN
0306-4522[print], 0306-4522[linking]
Statut éditorial
Publié
Date de publication
1987
Volume
23
Numéro
3
Pages
969-979
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
Loyez' myelin stain sometimes impregnates the perikarya and processes of neurons. This is rare in normal adult mice, but can be provoked by various means including electrical stimulation and mild ischaemia shortly before fixation. Passing a direct current or a train of pulses across the dura of the cerebral cortex produces a patch at the site of stimulation in which the neurons, but not the glia, are Loyez-impregnated; pulse-trains evoke such impregnation even at remote sites, presumably through orthodromic and/or antidromic activation. The strength and duration of stimulation necessary to provoke impregnation are below the threshold for causing an overt lesion. Local ischaemia produced by irreversibly occluding a small subarachnoid artery for as little as 7 min evokes a small patch of impregnated neurons in the superficial layers adjacent to the occluded artery. After slightly longer-lasting (e.g. 17 min) ischaemia, the impregnated zone spans most of the cortical depth. Mild global ischaemia produced by irreversibly occluding one or both common carotid arteries 30 min before fixation causes large numbers of neurons to be impregnated in many parts of the brain. That interventions as different as electrical stimulation and arterial occlusion both lead to neuronal impregnation may most plausibly be explained by the fact that they both cause cells to be depolarized. The method will be useful for visualizing overstimulated or ischaemic neurons, and may be applicable to the tracing of neural pathways.
Mots-clé
Animals, Brain/metabolism, Brain/pathology, Brain Ischemia/metabolism, Brain Ischemia/pathology, Electric Stimulation, Ischemic Attack, Transient/metabolism, Ischemic Attack, Transient/pathology, Mice, Mice, Inbred ICR, Myelin Sheath/analysis, Myelin Sheath/physiopathology, Postmortem Changes, Staining and Labeling/methods
Pubmed
Web of science
Création de la notice
20/01/2008 17:49
Dernière modification de la notice
20/08/2019 16:20
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