The effect of antigen concentration, antibody valency and size, and tumor architecture on antibody binding in multicell spheroids

Détails

ID Serval
serval:BIB_F311F9B60003
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The effect of antigen concentration, antibody valency and size, and tumor architecture on antibody binding in multicell spheroids
Périodique
International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology
Auteur⸱e⸱s
Langmuir  V. K., McGann  J. K., Buchegger  F., Sutherland  R. M.
ISSN
0969-8051
0883-2897 (Print)
Statut éditorial
Publié
Date de publication
1991
Volume
18
Numéro
7
Pages
753-64
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
Intact IgG1 and F(ab')2 anti-carcinoembryonic antigen antibodies penetrate human colon adenocarcinoma multicell spheroids much more slowly than Fab fragments and the molecular weight and the binding site valency appear to be the most important factor in determining the rate of penetration. The rate is also influenced considerably by the number of antigen binding sites per cell, with a high antigen concentration slowing penetration appreciably. The tumor cell architecture appears to have a minor effect on antibody penetration when compared to antibody size or antigen concentration.
Mots-clé
Adenocarcinoma/*pathology Antibodies, Neoplasm/*analysis/immunology Antigens, Neoplasm/*analysis/immunology Autoradiography Carcinoembryonic Antigen/immunology Colonic Neoplasms/*pathology Humans Immunoglobulin Fab Fragments/immunology Immunoglobulin G/immunology Microscopy, Electron Microspheres Tumor Cells, Cultured
Pubmed
Création de la notice
25/01/2008 11:28
Dernière modification de la notice
20/08/2019 16:20
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