Attempts to quantitate immunocytochemistry at the electron microscope level

Détails

ID Serval
serval:BIB_F2E863353440
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Attempts to quantitate immunocytochemistry at the electron microscope level
Périodique
Histochemical Journal
Auteur⸱e⸱s
Kraehenbuhl  J. P., Racine  L., Griffiths  G. W.
ISSN
0018-2214 (Print)
Statut éditorial
Publié
Date de publication
05/1980
Volume
12
Numéro
3
Pages
317-32
Notes
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S. --- Old month value: May
Résumé
The ability to localize intracellular macromolecules in situ by high resolution techniques has been made possible by the development of antibody labelling of thin sections obtained either from tissues embedded in an hydrophilic matrix, or by ultracryotomy or from conventional plastic embedded tissue. When particle-tagged immunological reagents are used to visualize intracellular antigens, quantitative information can be obtained by combining particle counts with morphometric estimations of compartment volume. Various detection systems have been used successfully for quantitation, which include ferritin-conjugated antibodies, biotin-avidin-ferritin complexes and, more recently, gold-protein A conjugates. Examples of the use of these techniques the localization of secretory proteins in pancreatic exocrine cells, opsin and a large membrane protein in photoreceptor cells of frog retina, and contractile proteins in skeletal muscle are given. Quantitative data obtained by morphometric analysis, both in bovine and rat pancreatic exocrine cells, are compared with values assessed by biochemical methods.
Mots-clé
Amylases/metabolism Animals Fixatives Histocytochemistry/*methods Immunochemistry/*methods Membrane Proteins/metabolism Microscopy, Electron/*methods Muscle Proteins/metabolism Muscles/ultrastructure Pancreas/metabolism Retina/metabolism Rhodopsin/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 15:06
Dernière modification de la notice
20/08/2019 16:20
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