Dynamics of multiple lin gene expression in Sphingomonas paucimobilis B90A in response to different hexachlorocyclohexane isomers.

Détails

ID Serval
serval:BIB_F279651A4881
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dynamics of multiple lin gene expression in Sphingomonas paucimobilis B90A in response to different hexachlorocyclohexane isomers.
Périodique
Applied and Environmental Microbiology
Auteur⸱e⸱s
Suar M., van der Meer J.R., Lawlor K., Holliger C., Lal R.
ISSN
0099-2240 (Print)
ISSN-L
0099-2240
Statut éditorial
Publié
Date de publication
2004
Volume
70
Numéro
11
Pages
6650-6656
Langue
anglais
Résumé
Sphingomonas paucimobilis B90A is able to degrade the alpha-, beta-, gamma-, and delta-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown S. paucimobilis B90A by RNA dot blot hybridization and real-time reverse transcriptase PCR upon exposure to a pulse of different HCH isomers. Irrespective of the addition of HCH, linA, linB, and linC were all expressed constitutively. In contrast, linD and linE were induced with alpha-HCH (2 mg/liter) and gamma-HCH (7 mg/liter). A sharp increase in mRNA levels for linD and linE was observed from 10 to 45 min after the addition of alpha- or gamma-HCH. Induction of linD and linE was not detectable upon the addition of 0.7 mg of gamma-HCH per liter, although the compound was degraded by the cells. The addition of beta-HCH (5 mg/liter) or delta-HCH (20 mg/liter) did not lead to linE and linD induction, despite the fact that 50% of the compounds were degraded. This suggests that degradation of beta- and delta-HCH proceeds by a different pathway than that of alpha- and gamma-HCH.
Mots-clé
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Biodegradation, Environmental, Culture Media, Gene Expression Regulation, Bacterial, Immunoblotting, Isomerism, Lindane/chemistry, Lindane/metabolism, Lyases/genetics, Lyases/metabolism, Nucleic Acid Hybridization, Reverse Transcriptase Polymerase Chain Reaction, Sphingomonas/genetics, Sphingomonas/growth & development
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 13:36
Dernière modification de la notice
20/08/2019 16:19
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