Modelling of crowded polymers elucidate effects of double-strand breaks in topological domains of bacterial chromosomes.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_F265DFDD4281
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Modelling of crowded polymers elucidate effects of double-strand breaks in topological domains of bacterial chromosomes.
Périodique
Nucleic Acids Research
Auteur(s)
Dorier J., Stasiak A.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
2013
Volume
41
Numéro
14
Pages
6808-6815
Langue
anglais
Résumé
Using numerical simulations of pairs of long polymeric chains confined in microscopic cylinders, we investigate consequences of double-strand DNA breaks occurring in independent topological domains, such as these constituting bacterial chromosomes. Our simulations show a transition between segregated and mixed state upon linearization of one of the modelled topological domains. Our results explain how chromosomal organization into topological domains can fulfil two opposite conditions: (i) effectively repulse various loops from each other thus promoting chromosome separation and (ii) permit local DNA intermingling when one or more loops are broken and need to be repaired in a process that requires homology search between broken ends and their homologous sequences in closely positioned sister chromatid.
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/09/2013 10:07
Dernière modification de la notice
20/08/2019 17:19
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