GRSF1 regulates RNA processing in mitochondrial RNA granules.

Détails

Ressource 1Télécharger: 23473034_BIB_F1AE1A701840.pdf (2224.53 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_F1AE1A701840
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
GRSF1 regulates RNA processing in mitochondrial RNA granules.
Périodique
Cell metabolism
Auteur⸱e⸱s
Jourdain A.A., Koppen M., Wydro M., Rodley C.D., Lightowlers R.N., Chrzanowska-Lightowlers Z.M., Martinou J.C.
ISSN
1932-7420 (Electronic)
ISSN-L
1550-4131
Statut éditorial
Publié
Date de publication
05/03/2013
Peer-reviewed
Oui
Volume
17
Numéro
3
Pages
399-410
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Various specialized domains have been described in the cytosol and the nucleus; however, little is known about compartmentalization within the mitochondrial matrix. GRSF1 (G-rich sequence factor 1) is an RNA binding protein that was previously reported to localize in the cytosol. We found that an isoform of GRSF1 accumulates in discrete foci in the mitochondrial matrix. These foci are composed of nascent mitochondrial RNA and also contain RNase P, an enzyme that participates in mitochondrial RNA processing. GRSF1 was found to interact with RNase P and to be required for processing of both classical and tRNA-less RNA precursors. In its absence, cleavage of primary RNA transcripts is abnormal, leading to decreased expression of mitochondrially encoded proteins and mitochondrial dysfunction. Our findings suggest that the foci containing GRSF1 and RNase P correspond to sites where primary RNA transcripts converge to be processed. We have termed these large ribonucleoprotein structures "mitochondrial RNA granules."
Mots-clé
Blotting, Northern, Bromodeoxyuridine, Gene Expression Regulation/genetics, Gene Expression Regulation/physiology, HEK293 Cells, Humans, Immunoprecipitation, Microscopy, Fluorescence, Mitochondria/metabolism, Poly(A)-Binding Proteins/metabolism, Protein Isoforms/metabolism, RNA/metabolism, RNA Interference, RNA Processing, Post-Transcriptional/physiology, RNA, Mitochondrial, Ribonuclease P/metabolism, Ribonucleoproteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/04/2021 17:25
Dernière modification de la notice
08/02/2022 7:36
Données d'usage