Gene transfer of the Ly-3 chain gene of the mouse CD8 molecular complex: co-transfer with the Ly-2 polypeptide gene results in detectable cell surface expression of the Ly-3 antigenic determinants.

Détails

ID Serval
serval:BIB_F06F752ADCC8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Gene transfer of the Ly-3 chain gene of the mouse CD8 molecular complex: co-transfer with the Ly-2 polypeptide gene results in detectable cell surface expression of the Ly-3 antigenic determinants.
Périodique
European Journal of Immunology
Auteur⸱e⸱s
Blanc D., Bron C., Gabert J., Letourneur F., MacDonald H.R., Malissen B.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
1988
Volume
18
Numéro
4
Pages
613-619
Langue
anglais
Résumé
The CD8 molecule is a glycoprotein expressed on a subset of mature T lymphocytes. It has been postulated to be a receptor for class I major histocompatibility complex molecules. In the mouse, CD8 is a heterodimer composed of Ly-2 and Ly-3 chains. We have isolated and analyzed cDNA and cosmid clones corresponding to the Ly-3 subunit. One of the isolated, cosmid clones was subsequently transfected, alone or in combination with the Ly-2 gene, into mouse Ltk- cells. Analysis of the Ly-2,3 molecules expressed at the surface of the double transfectants indicated that they are serologically and biochemically indistinguishable from their normal counterparts expressed on lymphoid cells. Ltk- cells transfected with the Ly-2 gene alone were shown to react with a subset of anti-CD8 monoclonal antibodies whereas Ly-3 transfectants did not stain with any of the anti-Ly-3 antibodies employed in this study. Since at least one of these antibodies (53-5.8) has been previously shown to recognize an epitope which is retained on the Ly-3 subunit after dissociation of the heterodimeric Ly-2,3 complex, these observations suggest that the expression of the Ly-2 polypeptide is required to permit the detectable cell surface expression of the antigenic determinants carried by the Ly-3 subunit.
Mots-clé
Amino Acid Sequence, Antigens, Differentiation, T-Lymphocyte/genetics, Antigens, Differentiation, T-Lymphocyte/immunology, Antigens, Ly/genetics, Antigens, Ly/immunology, Base Sequence, Chromosome Mapping, Cloning, Molecular, Cosmids, DNA/genetics, DNA Restriction Enzymes, Epitopes, Gene Expression Regulation, Molecular Sequence Data, Transfection
Pubmed
Web of science
Création de la notice
03/09/2011 20:05
Dernière modification de la notice
20/08/2019 16:18
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