T cell receptor genes in a series of class I major histocompatibility complex-restricted cytotoxic T lymphocyte clones specific for a Plasmodium berghei nonapeptide: implications for T cell allelic exclusion and antigen-specific repertoire
Détails
ID Serval
serval:BIB_EE4793509AA6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
T cell receptor genes in a series of class I major histocompatibility complex-restricted cytotoxic T lymphocyte clones specific for a Plasmodium berghei nonapeptide: implications for T cell allelic exclusion and antigen-specific repertoire
Périodique
Journal of Experimental Medicine
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
12/1991
Volume
174
Numéro
6
Pages
1371-83
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 1
Research Support, Non-U.S. Gov't --- Old month value: Dec 1
Résumé
We report here the first extensive study of a T cell repertoire for a class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocyte (CTL) response. We have found that the T cell receptors (TCRs) carried by 28 H-2Kd-restricted CTL clones specific for a single Plasmodium berghei circumsporozoite nonapeptide are highly diverse in terms of V alpha, J alpha, and J beta segments and aminoacid composition of the junctional regions. However, despite this extensive diversity, a high proportion of the TCRs contain the same V beta segment. These results are in contrast to most previously reported T cell responses towards class II MHC-peptide complexes, where the TCR repertoires appeared to be much more limited. In our study, the finding of a dominant V beta in the midst of otherwise highly diverse TCRs suggests the importance of the V beta segment in shaping the T cell repertoire specific for a given MHC-peptide complex. As an additional finding, we observed that nearly all clones have rearranged both TCR alpha loci. Moreover, as many as one-third of the CTL clones that we analyzed apparently display two productive alpha rearrangements. This argues against a regulated model of sequential recombination at the alpha locus and consequently raises the question of whether allelic exclusion of the TCR alpha chain is achieved at all.
Mots-clé
*Alleles
Amino Acid Sequence
Animals
Base Sequence
Clone Cells
Gene Rearrangement, T-Lymphocyte
H-2 Antigens/*immunology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Plasmodium berghei/*immunology
Protozoan Proteins/*immunology
Receptors, Antigen, T-Cell, alpha-beta/chemistry/*genetics
T-Lymphocytes, Cytotoxic/*immunology
Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:28
Dernière modification de la notice
20/08/2019 17:15