Keratinocyte growth factor (KGF) decreases ICAM-1 and VCAM-1 cell expression on bronchial epithelial cells.

Détails

ID Serval
serval:BIB_EDBE345F9782
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Keratinocyte growth factor (KGF) decreases ICAM-1 and VCAM-1 cell expression on bronchial epithelial cells.
Périodique
Clinical and experimental immunology
Auteur⸱e⸱s
Just N., Tillie-Leblond I., Guery B.P., Fourneau C., Tonnel A.B., Gosset P.
ISSN
0009-9104 (Print)
ISSN-L
0009-9104
Statut éditorial
Publié
Date de publication
04/2003
Peer-reviewed
Oui
Volume
132
Numéro
1
Pages
61-69
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Activation of leucocytes during airway inflammatory reaction involves adhesion to bronchial epithelial cells (BEC), a process implicating specific interactions between glycoproteins with epithelial cell surface proteins, mainly intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). In this study, the effect of keratinocyte growth factor (KGF), a growth factor involved in pulmonary epithelium repair, was evaluated on adhesion molecule expression with BEAS-2B cells and BEC and granulocyte adherence to BEAS-2B. The modulation by KGF of membrane and mRNA expression of ICAM-1 and VCAM-1 was studied on confluent cells stimulated or not with tumour necrosis factor-alpha (TNF) (200 UI/ml) or TNF and interleukin (IL)-4 (50 UI/ml and 10 ng/ml). Levels of soluble-(s)ICAM-1 and sVCAM-1 were measured by ELISA. Although moderately, KGF significantly decreased membrane ICAM-1 expression in unstimulated BEAS-2B cells (24% inhibition at 100 ng/ml) or in TNF- or TNF + IL-4-stimulated cells (22.5 and 18.7% inhibition, respectively). Treatment with KGF tended to decrease VCAM-1 expression in TNF- and TNF + IL-4-stimulated BEAS-2B (P = n.s. and P < 0.05, 14 and 15% inhibition, respectively). In primary culture of BEC, adhesion molecule expression was also reduced. ICAM-1 and VCAM-1 mRNA expression were also inhibited by KGF. Levels of sICAM-1 and sVCAM-1 were not significantly increased in supernatants from KGF-treated cells (30% and 24% increase at 100 ng/ml, respectively) compared to controls. Moreover, KGF decreased by 31% the adherence of neutrophils to TNF-activated BEAS-2B. In conclusion, KGF decreases ICAM-1 and VCAM-1 expression and neutrophil adherence in BEC. These suggest its involvement in the resolution of the inflammatory reaction.
Mots-clé
Bronchi/immunology, Cell Adhesion/drug effects, Cell Line, Epithelial Cells/drug effects, Epithelial Cells/immunology, Fibroblast Growth Factor 7, Fibroblast Growth Factors/pharmacology, Flow Cytometry, Humans, Intercellular Adhesion Molecule-1/analysis, Intercellular Adhesion Molecule-1/genetics, Interleukin-4/pharmacology, Neutrophils/cytology, RNA, Messenger/analysis, Reverse Transcriptase Polymerase Chain Reaction, Statistics, Nonparametric, Tumor Necrosis Factor-alpha/pharmacology, Vascular Cell Adhesion Molecule-1/analysis, Vascular Cell Adhesion Molecule-1/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/04/2021 10:59
Dernière modification de la notice
17/07/2023 14:29
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