Increased Presence of FOXP3+ Regulatory T Cells in Inflamed Muscle of Patients with Active Juvenile Dermatomyositis Compared to Peripheral Blood.

Détails

Ressource 1Télécharger: BIB_ED49FCF59722.P001.pdf (1332.84 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_ED49FCF59722
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Increased Presence of FOXP3+ Regulatory T Cells in Inflamed Muscle of Patients with Active Juvenile Dermatomyositis Compared to Peripheral Blood.
Périodique
Plos One
Auteur(s)
Vercoulen Y., Bellutti Enders F., Meerding J., Plantinga M., Elst E.F., Varsani H., van Schieveen C., Bakker M.H., Klein M., Scholman R.C., Spliet W., Ricotti V., Koenen H.J., de Weger R.A., Wedderburn L.R., van Royen-Kerkhof A., Prakken B.J.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
9
Numéro
8
Pages
e105353
Langue
anglais
Notes
Publication types: Journal Article Publication Status: epublish
Résumé
Juvenile dermatomyositis (JDM) is an immune-mediated inflammatory disease affecting the microvasculature of skin and muscle. CD4+CD25+FOXP3+ regulatory T cells (Tregs) are key regulators of immune homeostasis. A role for Tregs in JDM pathogenesis has not yet been established. Here, we explored Treg presence and function in peripheral blood and muscle of JDM patients. We analyzed number, phenotype and function of Tregs in blood from JDM patients by flow cytometry and in vitro suppression assays, in comparison to healthy controls and disease controls (Duchenne's Muscular Dystrophy). Presence of Tregs in muscle was analyzed by immunohistochemistry. Overall, Treg percentages in peripheral blood of JDM patients were similar compared to both control groups. Muscle biopsies of new onset JDM patients showed increased infiltration of numbers of T cells compared to Duchenne's muscular dystrophy. Both in JDM and Duchenne's muscular dystrophy the proportion of FOXP3+ T cells in muscles were increased compared to JDM peripheral blood. Interestingly, JDM is not a self-remitting disease, suggesting that the high proportion of Tregs in inflamed muscle do not suppress inflammation. In line with this, peripheral blood Tregs of active JDM patients were less capable of suppressing effector T cell activation in vitro, compared to Tregs of JDM in clinical remission. These data show a functional impairment of Tregs in a proportion of patients with active disease, and suggest a regulatory role for Tregs in JDM inflammation.
Pubmed
Web of science
Open Access
Oui
Création de la notice
02/10/2014 18:30
Dernière modification de la notice
20/08/2019 17:15
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