Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA.
Détails
ID Serval
serval:BIB_ED22B290CD8E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA.
Périodique
Cell Reports
ISSN
2211-1247 (Electronic)
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
14
Numéro
8
Pages
2003-2016
Langue
anglais
Résumé
Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of the Smc ATPase activity in the recruitment of Smc/ScpAB to the Bacillus subtilis chromosome. We demonstrate that targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. We find that dimerization of the Smc hinge domain stabilizes closed Smc rods and hinders head engagement as well as chromosomal targeting. Conversely, the ScpAB sub-complex promotes head engagement and Smc rod opening and thereby facilitates recruitment of Smc to parS sites. Upon ATP hydrolysis, Smc/ScpAB is released from loading sites and relocates within the chromosome-presumably through translocation along DNA double helices. Our findings define an intermediate state in the process of chromosome organization by Smc.
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/08/2016 10:36
Dernière modification de la notice
20/08/2019 17:15