Cardiopoietic programming of embryonic stem cells for tumor-free heart repair.

Détails

ID Serval
serval:BIB_ECFD2A3FCF95
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cardiopoietic programming of embryonic stem cells for tumor-free heart repair.
Périodique
Journal of Experimental Medicine
Auteur(s)
Behfar A., Perez-Terzic C., Faustino R.S., Arrell D.K., Hodgson D.M., Yamada S., Puceat M., Niederländer N., Alekseev A.E., Zingman L.V., Terzic A.
ISSN
0022-1007[print], 0022-1007[linking]
Statut éditorial
Publié
Date de publication
2007
Volume
204
Numéro
2
Pages
405-420
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Embryonic stem cells have the distinct potential for tissue regeneration, including cardiac repair. Their propensity for multilineage differentiation carries, however, the liability of neoplastic growth, impeding therapeutic application. Here, the tumorigenic threat associated with embryonic stem cell transplantation was suppressed by cardiac-restricted transgenic expression of the reprogramming cytokine TNF-alpha, enhancing the cardiogenic competence of recipient heart. The in vivo aptitude of TNF-alpha to promote cardiac differentiation was recapitulated in embryoid bodies in vitro. The procardiogenic action required an intact endoderm and was mediated by secreted cardio-inductive signals. Resolved TNF-alpha-induced endoderm-derived factors, combined in a cocktail, secured guided differentiation of embryonic stem cells in monolayers produce cardiac progenitors termed cardiopoietic cells. Characterized by a down-regulation of oncogenic markers, up-regulation, and nuclear translocation of cardiac transcription factors, this predetermined population yielded functional cardiomyocyte progeny. Recruited cardiopoietic cells delivered in infarcted hearts generated cardiomyocytes that proliferated into scar tissue, integrating with host myocardium for tumor-free repair. Thus, cardiopoietic programming establishes a strategy to hone stem cell pluripotency, offering a tumor-resistant approach for regeneration.
Mots-clé
Animals, Cell Differentiation/physiology, DNA Primers, Embryonic Stem Cells/cytology, Embryonic Stem Cells/transplantation, Gene Expression Regulation, Heart/physiology, Mice, Mice, Transgenic, Microarray Analysis, Myocytes, Cardiac/cytology, Neoplasms/prevention & control, Regeneration/physiology, Stem Cell Transplantation/methods, Transcription Factors/metabolism, Tumor Necrosis Factor-alpha/metabolism
Pubmed
Open Access
Oui
Création de la notice
21/06/2010 10:34
Dernière modification de la notice
20/08/2019 16:14
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