Gene expression profiling reveals consistent differences between clinical samples of human leukaemias and their model cell lines.
Détails
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_EC3C318E4A60
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Gene expression profiling reveals consistent differences between clinical samples of human leukaemias and their model cell lines.
Périodique
British Journal of Haematology
ISSN
0007-1048 (Print)
ISSN-L
0007-1048
Statut éditorial
Publié
Date de publication
2006
Volume
135
Numéro
4
Pages
520-523
Langue
anglais
Résumé
Microarray gene expression profiles of fresh clinical samples of chronic myeloid leukaemia in chronic phase, acute promyelocytic leukaemia and acute monocytic leukaemia were compared with profiles from cell lines representing the corresponding types of leukaemia (K562, NB4, HL60). In a hierarchical clustering analysis, all clinical samples clustered separately from the cell lines, regardless of leukaemic subtype. Gene ontology analysis showed that cell lines chiefly overexpressed genes related to macromolecular metabolism, whereas in clinical samples genes related to the immune response were abundantly expressed. These findings must be taken into consideration when conclusions from cell line-based studies are extrapolated to patients.
Mots-clé
Gene Expression, Gene Expression Profiling/methods, Humans, Leukemia/genetics, Leukemia/metabolism, Leukemia, Monocytic, Acute/genetics, Leukemia, Monocytic, Acute/metabolism, Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics, Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism, Leukemia, Myeloid/genetics, Leukemia, Promyelocytic, Acute/genetics, Leukemia, Promyelocytic, Acute/metabolism, Tumor Cells, Cultured/metabolism, Up-Regulation
Pubmed
Web of science
Création de la notice
22/04/2013 9:15
Dernière modification de la notice
20/08/2019 17:14