Gene expression in cultured endometrium from women with different outcomes following IVF.

Détails

ID Serval
serval:BIB_EB8EAFACCE7D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Gene expression in cultured endometrium from women with different outcomes following IVF.
Périodique
Molecular Human Reproduction
Auteur⸱e⸱s
Bersinger N.A., Wunder D.M., Birkhäuser M.H., Mueller M.D.
ISSN
1460-2407[electronic]
Statut éditorial
Publié
Date de publication
2008
Volume
14
Numéro
8
Pages
475-484
Langue
anglais
Résumé
Estradiol and progesterone are crucial for the acquisition of receptivity and the change in transcriptional activity of target genes in the implantation window. The aim of this study was to differentiate the regulation of genes in the endometrium of patients with recurrent implantation failure (IF) versus those who became pregnant after in vitro fertilization (IVF) treatment. Moreover, the effect of embryo-derived factors on endometrial transcriptional activity was studied. Nine women with known IVF outcome (IF, M, miscarriage, OP, ongoing pregnancy) and undergoing hysteroscopy with endometrial biopsy were enrolled. Biopsies were taken during the midluteal phase. After culture in the presence of embryo-conditioned IVF media, total RNA was extracted and submitted to reverse transcription, target cDNA synthesis, biotin labelling, fragmentation and hybridization using the Affymetrix Human Genome U133A 2.0 Chip. Differential expression of selected genes was re-analysed by quantitative PCR, in which the results were calculated as threshold cycle differences between the groups and normalized to Glyceraldehyde phosphate dehydrogenase and beta-actin. Differences were seen for several genes from endometrial tissue between the IF and the pregnancy groups, and when comparing OP with M, 1875 up- and 1807 down-regulated genes were returned. Real-time PCR analysis confirmed up-regulation for somatostatin, PLAP-2, mucin 4 and CD163, and down-regulation of glycodelin, IL-24, CD69, leukaemia inhibitory factor and prolactin receptor between Op and M. When the different embryo-conditioned media were compared, no significant differential regulation could be demonstrated. Although microarray profiling may currently not be sensitive enough for studying the effects of embryo-derived factors on the endometrium, the observed differences in gene expression between M and OP suggest that it will become an interesting tool for the identification of fertility-relevant markers produced by the endometrium.
Mots-clé
Embryo Implantation, Endometrium, Female, Fertilization in Vitro, Gene Expression Profiling, Humans, Oligonucleotide Array Sequence Analysis, Pregnancy, Pregnancy Outcome, Reverse Transcriptase Polymerase Chain Reaction, Tissue Culture Techniques
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/03/2009 20:09
Dernière modification de la notice
20/08/2019 17:13
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