T-cell stimulation with purified mycobacterial antigens in patients and healthy subjects infected with Mycobacterium leprae: secreted antigen 85 is another immunodominant antigen.

Détails

ID Serval
serval:BIB_EA36738C98FE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
T-cell stimulation with purified mycobacterial antigens in patients and healthy subjects infected with Mycobacterium leprae: secreted antigen 85 is another immunodominant antigen.
Périodique
Scandinavian Journal of Immunology
Auteur⸱e⸱s
Launois P., Niang M.N., Sarthou J.L., Rivier F., Drowart A., Van Vooren J.P., Millan J., Huygen K.
ISSN
0300-9475 (Print)
ISSN-L
0300-9475
Statut éditorial
Publié
Date de publication
1993
Volume
38
Numéro
2
Pages
167-176
Langue
anglais
Résumé
Peripheral blood leucocytes from 9 paucibacillary and 12 multibacillary leprosy patients, from 18 healthy controls and from 34 healthy leprosy contacts were stimulated with three mycobacterial heat shock proteins with respective molecular weights of 70, 65 and 18 kDa and with the secreted 30-32 kDa protein, also called antigen 85. Antigen 85 was found to be the most powerful T-cell antigen (as measured by lymphoproliferation and IFN-gamma secretion), eliciting a positive response in all (100%) paucibacillary patients and in all lepromin-positive controls and contacts. The three heat shock proteins (hsp) were less active T-cell stimuli. Reactivity to the 70 kDa hsp was found in only 44% of the paucibacillary patients, in 80% of the lepromin-positive controls and in 60% of the lepromin-positive leprosy contacts. The 65 kDa hsp stimulated T cells in 89% of the paucibacillary patients and in 80% of the lepromin-positive controls and contacts. Responsiveness to the 18 kDa hsp, finally, was clearly more frequent in tuberculoid leprosy patients (78%) than in lepromin-positive controls (40%) or lepromin-positive leprosy contacts (4%). T-cell reactivity of 8 lepromin-negative controls, of 9 lepromin-negative contacts and of 12 multibacillary leprosy patients was low to all the antigens tested. Although proliferative and IFN-gamma responses were generally closely related, some subjects demonstrated a dissociation of these two immune parameters. Our data confirm previous findings on the powerful T-cell stimulatory properties of antigen 85 during M. leprae infection and suggest that this antigen is indeed a potentially protective T-cell immunogen.
Mots-clé
Antigens, Bacterial/immunology, Bacterial Proteins/immunology, Heat-Shock Proteins/immunology, Humans, Interferon-gamma/physiology, Leprosy/immunology, Lymphocyte Activation, Mycobacterium bovis/immunology, Mycobacterium leprae/immunology, T-Lymphocytes/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 11:06
Dernière modification de la notice
20/08/2019 16:12
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