Comparison of lactate transport in astroglial cells and monocarboxylate transporter 1 (MCT 1) expressing Xenopus laevis oocytes. Expression of two different monocarboxylate transporters in astroglial cells and neurons
Détails
ID Serval
serval:BIB_E9E2E29BA359
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparison of lactate transport in astroglial cells and monocarboxylate transporter 1 (MCT 1) expressing Xenopus laevis oocytes. Expression of two different monocarboxylate transporters in astroglial cells and neurons
Périodique
Journal of Biological Chemistry
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
11/1997
Peer-reviewed
Oui
Volume
272
Numéro
48
Pages
30096-102
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 28
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 28
Résumé
The transport of lactate is an essential part of the concept of metabolic coupling between neurons and glia. Lactate transport in primary cultures of astroglial cells was shown to be mediated by a single saturable transport system with a Km value for lactate of 7.7 mM and a Vmax value of 250 nmol/(min x mg of protein). Transport was inhibited by a variety of monocarboxylates and by compounds known to inhibit monocarboxylate transport in other cell types, such as alpha-cyano-4-hydroxycinnamate and p-chloromercurbenzenesulfonate. Using reverse transcriptase-polymerase chain reaction and Northern blotting, the presence of mRNA coding for the monocarboxylate transporter 1 (MCT1) was demonstrated in primary cultures of astroglial cells. In contrast, neuron-rich primary cultures were found to contain the mRNA coding for the monocarboxylate transporter 2 (MCT2). MCT1 was cloned and expressed in Xenopus laevis oocytes. Comparison of lactate transport in MCT1 expressing oocytes with lactate transport in glial cells revealed that MCT1 can account for all characteristics of lactate transport in glial cells. These data provide further molecular support for the existence of a lactate shuttle between astrocytes and neurons.
Mots-clé
Animals
Astrocytes/*metabolism
Biological Transport/drug effects
Carrier Proteins/*metabolism
Cells, Cultured
Gene Expression
Kinetics
Lactates/*metabolism
Monocarboxylic Acid Transporters
Neurons/*metabolism
Oocytes
Pyruvates/metabolism
RNA, Messenger/genetics
Rats
Rats, Wistar
Xenopus laevis
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/01/2008 19:22
Dernière modification de la notice
20/08/2019 17:12