Evaluation of hepatic and whole body glycogen metabolism in humans during repeated administrations of small loads of 13C glucose

Détails

ID Serval
serval:BIB_E8B4F971BEA8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evaluation of hepatic and whole body glycogen metabolism in humans during repeated administrations of small loads of 13C glucose
Périodique
Diabetes and Metabolism
Auteur(s)
Selz  R., Theintz  G., Tappy  L., Schneiter  P.
ISSN
1262-3636 (Print)
Statut éditorial
Publié
Date de publication
12/2003
Volume
29
Numéro
6
Pages
643-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
BACKGROUND: Postprandial suppression of endogenous glucose production and regulation of glucose homeostasis involve alterations of whole body and hepatic glycogenolysis and glycogen breakdown. These parameters can be estimated by the simultaneous measurement of net total and exogenous, (13)C-labeled, glucose oxidation. METHODS: Eight subjects were studied on 3 occasions, while receiving oral loads of 60 mg, 120 or 180 mg (13)C glucose/kg every hour for 4 consecutive hours. Net glucose oxidation was calculated from indirect calorimetry, and exogenous glucose oxidation from (13)CO(2) production. These parameters were evaluated during the hour following the fourth glucose load. Whole body endogenous glycogen breakdown was calculated as (net glucose oxidation) - (exogenous glucose oxidation). Total glycogen synthesis was calculated as (glucose load) - (exogenous glucose oxidation). Whole body glucose turnover was measured with 6.6 (2)H(2) glucose. The systemic appearance of oral, (13)C labeled glucose was monitored, and the suppression of endogenous glucose production was calculated. RESULTS: Plasma glucose tracers had reached near steady state during the hour following the fourth glucose load. Glucose ingestion dose-dependently suppressed endogenous glycogen breakdown and stimulated total glycogen synthesis. Endogenous glycogen breakdown was completely inhibited with 180 mg oral glucose/kg. Endogenous glucose production was suppressed in a dose-dependent way, but remained positive with all 3 doses. The first pass splanchnic glucose uptake averaged 25-35%. CONCLUSION: Repeated administration of small doses of (13)C labeled glucose allow to reach near steady state conditions after four hours, and to non-invasively evaluate whole body glycogen turnover and hepatic glucose metabolism.
Mots-clé
Adult Blood Glucose/analysis Calorimetry, Indirect Carbon Isotopes/*diagnostic use Deuterium/diagnostic use Dose-Response Relationship, Drug Fatty Acids, Nonesterified/blood Glucagon/blood Glucose/*administration & dosage/diagnostic use/metabolism Glycogen/biosynthesis/*metabolism Humans Insulin/blood Kinetics Liver/*metabolism Male Oxidation-Reduction
Pubmed
Web of science
Création de la notice
24/01/2008 14:37
Dernière modification de la notice
20/08/2019 17:11
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