Establishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules.

Détails

Ressource 1Télécharger: BIB_E87051F5395A.P001.pdf (20308.37 [Ko])
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_E87051F5395A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Establishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules.
Périodique
Plos Genetics
Auteur⸱e⸱s
Bieli D., Kanca O., Requena D., Hamaratoglu F., Gohl D., Schedl P., Affolter M., Slattery M., Müller M., Estella C.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
11
Numéro
10
Pages
e1005376
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Résumé
The subdivision of cell populations in compartments is a key event during animal development. In Drosophila, the gene apterous (ap) divides the wing imaginal disc in dorsal vs ventral cell lineages and is required for wing formation. ap function as a dorsal selector gene has been extensively studied. However, the regulation of its expression during wing development is poorly understood. In this study, we analyzed ap transcriptional regulation at the endogenous locus and identified three cis-regulatory modules (CRMs) essential for wing development. Only when the three CRMs are combined, robust ap expression is obtained. In addition, we genetically and molecularly analyzed the trans-factors that regulate these CRMs. Our results propose a three-step mechanism for the cell lineage compartment expression of ap that includes initial activation, positive autoregulation and Trithorax-mediated maintenance through separable CRMs.
Mots-clé
Animals, Body Patterning/genetics, Cell Lineage, Drosophila Proteins/biosynthesis, Drosophila Proteins/genetics, Drosophila melanogaster/genetics, Drosophila melanogaster/growth & development, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Homeodomain Proteins/biosynthesis, Homeodomain Proteins/genetics, Imaginal Discs/growth & development, LIM-Homeodomain Proteins/biosynthesis, LIM-Homeodomain Proteins/genetics, Regulatory Elements, Transcriptional/genetics, Regulatory Sequences, Nucleic Acid/genetics, Transcription Factors/biosynthesis, Transcription Factors/genetics, Wings, Animal/growth & development
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/12/2015 11:13
Dernière modification de la notice
20/08/2019 17:11
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