Plasma determination of flumazenil, a benzodiazepine antagonist, by immunotoxicology and by capillary gas chromatography/mass spectrometry.

Détails

ID Serval
serval:BIB_E8207BC19E85
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Plasma determination of flumazenil, a benzodiazepine antagonist, by immunotoxicology and by capillary gas chromatography/mass spectrometry.
Périodique
Journal of Analytical Toxicology
Auteur(s)
Kintz P., Mangin P.
ISSN
0146-4760[print], 0146-4760[linking]
Statut éditorial
Publié
Date de publication
1991
Volume
15
Numéro
4
Pages
202-203
Langue
anglais
Résumé
The method presented describes a sensitive and specific quantitative assay for the determination of flumazenil in human plasma. Flumazenil and an internal standard, midazolam, are isolated by a basic extraction. The final extract is separated on a 25-m BP-1 capillary column and drugs are detected by selected ion monitoring at m/z 229 and m/z 310 for flumazenil and the internal standard, respectively. The minimum detectable quantity is 1.0 ng/mL, for flumazenil in plasma. Coefficients of variation for within-run data were less than 6%.
Mots-clé
Flumazenil/analysis, Gas Chromatography-Mass Spectrometry, Humans, Hydrogen-Ion Concentration, Reproducibility of Results, Solvents
Pubmed
Création de la notice
15/10/2010 15:17
Dernière modification de la notice
20/08/2019 16:10
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