Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae.

Détails

ID Serval
serval:BIB_E7A09848DB60
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae.
Périodique
Journal of Biological Chemistry
Auteur(s)
Fankhauser C., Homans S.W., Thomas-Oates J.E., McConville M.J., Desponds C., Conzelmann A., Ferguson M.A.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
12/1993
Peer-reviewed
Oui
Volume
268
Numéro
35
Pages
26365-26374
Langue
anglais
Résumé
Metabolic labeling studies suggest that Saccharomyces cerevisiae contains many glycoproteins that are anchored in the lipid bilayer by glycosylphosphatidylinositol membrane anchors. Membrane anchors were purified from a crude yeast membrane protein fraction and analyzed by two-dimensional 1H-1H NMR, fast atom bombardment-mass spectrometry, compositional and methylation linkage analyses, as well as chemical and enzymatic modifications. The yeast glycosylphosphatidylinositol anchors consist of the following structures: ethanolamine-PO4-6(R-2)Man alpha 1-2Man alpha 1-6Man alpha 1-4Glc-NH2 alpha 1-6myo-inositol-1-PO4-lipid, where R is mainly Man alpha 1- (80%) with some Man alpha 1-2Man alpha 1- (15%) and Man alpha 1-3Man alpha 1- (5%). The core region of the yeast anchors (ethanolamine-PO4-6Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcNH2 alpha 1-6myo-inositol-1-PO4) is identical to the conserved core region found in glycosylphosphatidylinositol anchors from protozoa and mammals. The lipid moieties of the total yeast glycosylphosphatidylinositol anchors are mainly ceramides, consisting mostly of C18:0 phytosphingosine and C26:0 fatty acid. However, the lipid moiety of the glycosylphosphatidylinositol anchor of the purified ggp125 protein is a lyso- or diacylglycerol, containing C26:0 fatty acids. This suggests that yeast adds different lipid components to the glycosylphosphatidylinositol anchors of different proteins.
Mots-clé
Carbohydrate Conformation, Carbohydrate Sequence, Fungal Proteins/chemistry, Gas Chromatography-Mass Spectrometry, Glycolipids/chemistry, Glycopeptides/chemistry, Glycosylphosphatidylinositols/chemistry, Lipids/chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Saccharomyces cerevisiae/chemistry, Spectrometry, Mass, Fast Atom Bombardment
Pubmed
Web of science
Création de la notice
24/01/2008 15:29
Dernière modification de la notice
20/08/2019 16:10
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