Evaluation of internal transcribed spacer region of ribosomal DNA sequence analysis for molecular characterization of Candida albicans and Candida dubliniensis isolates from HIV-infected patients
Détails
ID Serval
serval:BIB_E75312B2FB2F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evaluation of internal transcribed spacer region of ribosomal DNA sequence analysis for molecular characterization of Candida albicans and Candida dubliniensis isolates from HIV-infected patients
Périodique
Medical Mycology
ISSN
1369-3786 (Print)
Statut éditorial
Publié
Date de publication
12/2002
Volume
40
Numéro
6
Pages
535-43
Notes
Comparative Study
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
Molecular typing systems have been needed to study Candida colonization in HIV-infected patients, particularly for investigating virulence and fluconazole resistance. Three methods--electrophoretic karyotyping (EK), detection of restriction fragment length polymorphisms (RFLP) and randomly amplified polymorphic DNA analysis (RAPD)--have been most frequently used. In this study, comparative sequence analysis of the internal transcribed spacer (ITS) region of rDNA was evaluated for delineation of Candida isolates from 14 HIV-infected patients. EK, ITS sequence analysis, RFLP and RAPD resulted in 11, 10, 9 and 8 DNA genotypes, respectively, from 39 Candida albicans isolates. The 10 genotypes observed using ITS sequence analysis were defined by six variation sites in the sequence. Molecular typing of sequential oral isolates showed the persistence of the same genotype of C. albicans in nine patients, and genotype variation in one patient. EK and RAPD showed that another patient was co-infected by two distinct genotypes and ITS analysis identified one of the two genotypes as Candida dubliniensis. Comparative ITS sequence analysis is a quick and reproducible method that provides clear and objective results, and it also identifies C. dubliniensis. The discriminatory power of this new typing approach could be improved by concomitant analysis of other DNA polymorphic sequences.
Mots-clé
AIDS-Related Opportunistic Infections/microbiology
Candida/*classification/genetics
Candida albicans/*classification/genetics
Candidiasis, Oral/microbiology
DNA, Fungal/analysis
DNA, Ribosomal Spacer/analysis
HIV Infections/*complications
Humans
Karyotyping
Mycological Typing Techniques
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
Pubmed
Web of science
Création de la notice
25/01/2008 17:46
Dernière modification de la notice
20/08/2019 17:10