Analyse des réarrangements des gènes des chaînes lourdes des immunoglobulines par PCR sur tissu enrobé en paraffine: application aux lymphomes B en Tunisie [Analysis of immunoglobulin heavy chain genes rearrangement by PCR from paraffin-embedded tissue in B-cell lymphomes in Tunisia]
Détails
ID Serval
serval:BIB_E6FA118F68EC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Analyse des réarrangements des gènes des chaînes lourdes des immunoglobulines par PCR sur tissu enrobé en paraffine: application aux lymphomes B en Tunisie [Analysis of immunoglobulin heavy chain genes rearrangement by PCR from paraffin-embedded tissue in B-cell lymphomes in Tunisia]
Périodique
Annales de biologie clinique
ISSN
0003-3898 (Print)
ISSN-L
0003-3898
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
63
Numéro
1
Pages
75-81
Langue
français
Notes
Publication types: English Abstract ; Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
To study the lymphoid clonality on Tunisian B-cell lymphomas cases by polymerase chain reaction (PCR)-based techniques using DNA from paraffin-embedded tissues.
Here we conducted a retrospective PCR clonality study on 73 cases of B-cell lymphomas and 12 reactive lymphoid tissues. The quality of DNA extracted was tested by beta-globin PCR. Consensus primers directed at the FRIII-VH and FRII-VH regions of the immunoglobulin heavy chain (IgH) gene were used to detect clonality.
The results showed that 52 of 73 (71%) B-cell lymphomas exhibited good quality of amplifiable DNA. Clonality was found in 77% of cases using the set of primers FRIIIa/LJH/VLJH and in 65.5% using the set of primers FRIIa/LJH/VLJH. Lymphomas derived from pregerminal centre showed a high rate detection of clonal IgH gene rearrangement (100%) compared to other group of tumors derived from germinal centre or postgerminal centre (74.5%). None of the polyclonal controls gave a clonal pattern.
This is the first large series of PCR clonality study of IgH gene rearrangements on B-cell lymphoma from Tunisia. Our results were similar to other reports in terms of sensitivity and specificity of these techniques and confirm the interest of that PCR for detecting clonal IgH gene rearrangements in lymphoma.
Here we conducted a retrospective PCR clonality study on 73 cases of B-cell lymphomas and 12 reactive lymphoid tissues. The quality of DNA extracted was tested by beta-globin PCR. Consensus primers directed at the FRIII-VH and FRII-VH regions of the immunoglobulin heavy chain (IgH) gene were used to detect clonality.
The results showed that 52 of 73 (71%) B-cell lymphomas exhibited good quality of amplifiable DNA. Clonality was found in 77% of cases using the set of primers FRIIIa/LJH/VLJH and in 65.5% using the set of primers FRIIa/LJH/VLJH. Lymphomas derived from pregerminal centre showed a high rate detection of clonal IgH gene rearrangement (100%) compared to other group of tumors derived from germinal centre or postgerminal centre (74.5%). None of the polyclonal controls gave a clonal pattern.
This is the first large series of PCR clonality study of IgH gene rearrangements on B-cell lymphoma from Tunisia. Our results were similar to other reports in terms of sensitivity and specificity of these techniques and confirm the interest of that PCR for detecting clonal IgH gene rearrangements in lymphoma.
Mots-clé
Base Sequence, DNA Primers, DNA, Neoplasm/genetics, DNA, Neoplasm/isolation & purification, Gene Rearrangement, B-Lymphocyte, Heavy Chain/genetics, Globins/genetics, Humans, Immunoglobulin Heavy Chains/genetics, Lymphoma, B-Cell/genetics, Polymerase Chain Reaction, Sensitivity and Specificity, Tunisia
Pubmed
Web of science
Création de la notice
17/10/2023 9:54
Dernière modification de la notice
20/10/2023 6:10
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