Adenosine and ADP prevent apoptosis in cultured rat cerebellar granule cells

Détails

ID Serval
serval:BIB_E6925C55F5D9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Adenosine and ADP prevent apoptosis in cultured rat cerebellar granule cells
Périodique
Brain Research
Auteur(s)
Vitolo  O. V., Ciotti  M. T., Galli  C., Borsello  T., Calissano  P.
ISSN
0006-8993 (Print)
Statut éditorial
Publié
Date de publication
11/1998
Volume
809
Numéro
2
Pages
297-301
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 2
Résumé
Cerebellar granule cells (CGCs) explanted in vitro undergo death via apoptosis when the concentration of potassium is shifted from 25 mM to 5 mM. We report that adenosine and ADP, which act as neurotransmitters and neuromodulators in the brain, exert in cultured cerebellar granule cells a specific and marked antiapoptotic action with half-maximal effect in the 10-100 microM range. The action of adenosine is partly inhibited by the A1AR antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) and is mimicked by the A1AR agonist 2-chloro-N6-cyclopentyladenosine (CCPA), while ADP effect, that is completely blocked by the P2x, P2y receptors noncompetitive antagonist suramine, is restored in the presence of the selective P2x purinoceptors agonist beta, gamma-methylene-L-ATP. These findings demonstrate that adenosine and ADP markedly inhibit the program of cell death in cerebellar granule cells and suggest that such an action is mediated via interaction with, respectively, A1 and P2x receptors.
Mots-clé
Adenosine/analogs & derivatives/*pharmacology Adenosine Diphosphate/*pharmacology Animals Apoptosis/*drug effects Cells, Cultured Cerebellum/*cytology In Situ Nick-End Labeling Neurons/chemistry/cytology/metabolism Potassium Chloride/pharmacokinetics Rats Rats, Wistar Receptors, Purinergic/physiology Xanthines/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 14:19
Dernière modification de la notice
20/08/2019 16:09
Données d'usage