A rod conformation of the Pyrococcus furiosus Rad50 coiled coil.

Détails

ID Serval
serval:BIB_E5C92095B881
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A rod conformation of the Pyrococcus furiosus Rad50 coiled coil.
Périodique
Proteins
Auteur(s)
Soh Y.M., Basquin J., Gruber S.
ISSN
1097-0134 (Electronic)
ISSN-L
0887-3585
Statut éditorial
Publié
Date de publication
02/2021
Peer-reviewed
Oui
Volume
89
Numéro
2
Pages
251-255
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The Rad50-Mre11 nuclease complex plays a vital role in DNA repair in all domains of life. It recognizes and processes DNA double-strand breaks. Rad50 proteins fold into an extended structure with a 20 to 60 nm long coiled coil connecting a globular ABC ATPase domain with a zinc hook dimerization domain. A published structure of an archaeal Rad50 zinc hook shows coiled coils pointing away from each other. Here we present the crystal structure of an alternate conformation displaying co-aligned coiled coils. Archaeal Rad50 may thus switch between rod-shaped and ring-like conformations as recently proposed for a bacterial homolog.
Mots-clé
Amino Acid Motifs, Archaeal Proteins/chemistry, Archaeal Proteins/genetics, Archaeal Proteins/metabolism, Binding Sites, Cations, Divalent, Cloning, Molecular, Crystallography, X-Ray, DNA Repair, DNA, Archaeal/chemistry, DNA, Archaeal/genetics, DNA, Archaeal/metabolism, Endodeoxyribonucleases/chemistry, Endodeoxyribonucleases/genetics, Endodeoxyribonucleases/metabolism, Escherichia coli/genetics, Escherichia coli/metabolism, Exodeoxyribonucleases/chemistry, Exodeoxyribonucleases/genetics, Exodeoxyribonucleases/metabolism, Gene Expression, Genetic Vectors/chemistry, Genetic Vectors/metabolism, Humans, Models, Molecular, Protein Binding, Protein Conformation, alpha-Helical, Protein Interaction Domains and Motifs, Protein Multimerization, Pyrococcus furiosus/genetics, Pyrococcus furiosus/metabolism, Recombinant Proteins/chemistry, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Structural Homology, Protein, Zinc/chemistry, Zinc/metabolism, DNA repair, Mre11, Rad50, SMC, SMC-like, coiled coil, rod, zinc hook
Pubmed
Web of science
Création de la notice
15/09/2020 9:55
Dernière modification de la notice
01/06/2021 5:36
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