Gastric mucosal alpha(4)beta(7)-integrin-positive CD4 T lymphocytes and immune protection against helicobacter infection in mice

Détails

ID Serval
serval:BIB_E55C0ED4F5AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Gastric mucosal alpha(4)beta(7)-integrin-positive CD4 T lymphocytes and immune protection against helicobacter infection in mice
Périodique
Gastroenterology
Auteur(s)
Michetti  M., Kelly  C. P., Kraehenbuhl  J. P., Bouzourene  H., Michetti  P.
ISSN
0016-5085 (Print)
Statut éditorial
Publié
Date de publication
07/2000
Volume
119
Numéro
1
Pages
109-18
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul
Résumé
BACKGROUND & AIMS: The integrin alpha(4)beta(7) mediates homing of effector/memory lymphocytes to the intestine and the mucosa-associated lymphoid tissue. This study examined the ability of alpha(4)beta(7)(hi) CD4(+) T lymphocytes to home to the stomach and their role in immunization-mediated protection against Helicobacter felis infection. METHODS: Gastric lamina propria and circulating mononucleated cells of naive, infected, and immunized Swiss Webster mice were isolated, and alpha(4)beta(7)-integrin expression was quantified by flow cytometry on CD4(+) T lymphocytes. Anti-alpha(4)beta(7)-integrin antibody was used to block alpha(4)beta(7) function in vivo. RESULTS: In naive mice, alpha(4)beta(7)(hi) CD4(+) T cells were enriched approximately 10-fold in the gastric mucosa compared with peripheral blood (P<0.0001). Chronic H. felis infection did not alter these proportions, but oral immunization with H. felis sonicate plus cholera toxin (CT) or with CT alone markedly increased gastric alpha(4)beta(7)(hi) CD4(+) T cells compared with naive and infected controls (P = 0.0008 and P = 0.002 for H. felis sonicate and CT, respectively). Anti-alpha(4)beta(7)-integrin antibody blocked the protection induced by oral immunization with H. felis sonicate and CT. CONCLUSIONS: The integrin alpha(4)beta(7) participates in the homing of CD4(+) T lymphocytes to the stomach and in the protection of the gastric mucosa against H. felis infection.
Mots-clé
Administration, Oral Animals CD4-Positive T-Lymphocytes/metabolism/*physiology Female Gastric Mucosa/*metabolism/pathology Helicobacter/immunology Helicobacter Infections/metabolism/pathology/*prevention & control Immune System/*physiology Immunization Integrins/*metabolism Mice Receptors, Lymphocyte Homing/physiology
Pubmed
Web of science
Création de la notice
25/01/2008 16:02
Dernière modification de la notice
20/08/2019 16:08
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