Strong increase in the tyrosine phosphorylation of actin upon inhibition of oxidative phosphorylation: correlation with reversible rearrangements in the actin skeleton of Dictyostelium cells.

Détails

ID Serval
serval:BIB_E54904F41BB0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Strong increase in the tyrosine phosphorylation of actin upon inhibition of oxidative phosphorylation: correlation with reversible rearrangements in the actin skeleton of Dictyostelium cells.
Périodique
Journal of Cell Science
Auteur⸱e⸱s
Jungbluth A., von Arnim V., Biegelmann E., Humbel B., Schweiger A., Gerisch G.
ISSN
0021-9533 (Print)
ISSN-L
0021-9533
Statut éditorial
Publié
Date de publication
1994
Volume
107
Numéro
1
Pages
117-125
Langue
anglais
Résumé
When oxidative phosphorylation is inhibited in cells of Dictyostelium discoideum, the phosphorylation of tyrosine residues on actin is strongly increased. This increase is fully reversible. Under the same conditions the amoeboid cells undergo a series of shape changes. Within three minutes the pseudopods are withdrawn and replaced by cell surface blebs. Subsequently, the cells are rounding up to become immobile. In parallel with the changes in cell shape, the distribution of actin filaments is grossly altered within the cells. The cortical network of actin filaments of normal cells is broken down, and the F-actin forms large, irregular clusters deep within the cytoplasm. In these clusters the actin is associated with myosin II and with the heterodimeric F-actin capping protein cap32/34. After restoration of oxidative phosphorylation the actin returns within less than four minutes to its normal cortical position. A causal relationship between tyrosine phosphorylation and changes in the distribution of actin remains to be established. The rearrangements in the actin system that result from the inhibition of oxidative phosphorylation indicate that the organisation of this system and its maintenance in a functional state depend on the continuous supply of energy by ATP.
Mots-clé
Actins/metabolism, Adenosine Triphosphate/metabolism, Animals, Arsenicals/pharmacology, Cytoskeleton/metabolism, Cytoskeleton/ultrastructure, Dictyostelium/drug effects, Dictyostelium/metabolism, Dinitrophenols/pharmacology, Electrophoresis, Polyacrylamide Gel, Genistein, Growth Inhibitors/pharmacology, Isoflavones/pharmacology, Microscopy, Fluorescence, Oxidative Phosphorylation/drug effects, Phosphoproteins/isolation & purification, Phosphoproteins/metabolism, Phosphotyrosine, Protein-Tyrosine Kinases/antagonists & inhibitors, Protein-Tyrosine Kinases/metabolism, Tyrosine/analogs & derivatives, Tyrosine/analysis
Pubmed
Web of science
Création de la notice
18/10/2012 14:44
Dernière modification de la notice
20/08/2019 17:08
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