DNA segment capture by Smc5/6 holocomplexes.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_E4AD008053CC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
DNA segment capture by Smc5/6 holocomplexes.
Périodique
Nature structural & molecular biology
Auteur⸱e⸱s
Taschner M., Gruber S.
ISSN
1545-9985 (Electronic)
ISSN-L
1545-9985
Statut éditorial
Publié
Date de publication
05/2023
Peer-reviewed
Oui
Volume
30
Numéro
5
Pages
619-628
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Three distinct structural maintenance of chromosomes (SMC) complexes facilitate chromosome folding and segregation in eukaryotes, presumably by DNA loop extrusion. How SMCs interact with DNA to extrude loops is not well understood. Among the SMC complexes, Smc5/6 has dedicated roles in DNA repair and preventing a buildup of aberrant DNA junctions. In the present study, we describe the reconstitution of ATP-dependent DNA loading by yeast Smc5/6 rings. Loading strictly requires the Nse5/6 subcomplex which opens the kleisin neck gate. We show that plasmid molecules are topologically entrapped in the kleisin and two SMC subcompartments, but not in the full SMC compartment. This is explained by the SMC compartment holding a looped DNA segment and by kleisin locking it in place when passing between the two flanks of the loop for neck-gate closure. Related segment capture events may provide the power stroke in subsequent DNA extrusion steps, possibly also in other SMC complexes, thus providing a unifying principle for DNA loading and extrusion.
Mots-clé
Multiprotein Complexes/genetics, DNA/chemistry, Chromosomes/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, DNA Repair, Cell Cycle Proteins/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Chromosomal Proteins, Non-Histone/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/04/2023 9:28
Dernière modification de la notice
09/02/2024 8:54
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