Purified plasma factor XIIa aggregates human neutrophils and causes degranulation

Détails

ID Serval
serval:BIB_E46C638F9404
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Purified plasma factor XIIa aggregates human neutrophils and causes degranulation
Périodique
Blood
Auteur⸱e⸱s
Wachtfogel  Y. T., Pixley  R. A., Kucich  U., Abrams  W., Weinbaum  G., Schapira  M., Colman  R. W.
ISSN
0006-4971 (Print)
Statut éditorial
Publié
Date de publication
06/1986
Volume
67
Numéro
6
Pages
1731-7
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Résumé
Plasma kallikrein has been shown to aggregate human neutrophils and release human neutrophil elastase. However, neutrophils resuspended in factor XII-deficient plasma released only 30% of the elastase compared with normal plasma. Isolated human neutrophils were aggregated in a concentration-dependent fashion by 0.06 to 0.6 U/mL factor XIIa (0.022 to 0.22 mumol/L). Factor XIIa (0.1 to 1.0 U/mL) also induced neutrophil degranulation as evidenced by a concentration-dependent release of the specific granule protein, lactoferrin, and azurophilic granule protease, elastase. The release of neutrophil elastase was biphasic, reaching 40% of maximum at 15 seconds with maximal release by 90 minutes. The active site of factor XIIa was required, since the synthetic inhibitor, D-Pro-Phe-Arg-CH2Cl, which reacts with an essential histidine, and the natural plasma inhibitor, Cl-inhibitor, which interacts with the critical serine, both inhibit by more than 90% the release of elastase. The heavy chain is also required, since factor XII fragments failed to aggregate neutrophils or stimulate degranulation. Factor XIIa (0.6 U/mL) can completely correct the defect in elastase release evident in factor XII-deficient plasma. These studies demonstrate that factor XIIa, at concentrations potentially obtainable in plasma in disease states, can activate neutrophils, and thus may participate in the inflammatory response.
Mots-clé
Cell Aggregation/drug effects Enzyme-Linked Immunosorbent Assay Factor XII/*pharmacology Factor XIIa Humans Kinetics Lactoferrin/pharmacology Molecular Weight Neutrophils/*drug effects Pancreatic Elastase/blood Peptide Fragments/*pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 15:27
Dernière modification de la notice
20/08/2019 16:08
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