Indeterminate test results of T-SPOT.TB performed under routine field conditions

Détails

ID Serval
serval:BIB_E3AFCB0EF0F1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Indeterminate test results of T-SPOT.TB performed under routine field conditions
Périodique
European Respiratory Journal
Auteur⸱e⸱s
Beffa P., Zellweger A., Janssens J. P., Wrighton-Smith P., Zellweger J. P.
ISSN
1399-3003
Statut éditorial
Publié
Date de publication
2008
Volume
31
Numéro
4
Pages
842-846
Langue
anglais
Résumé
Interferon-gamma release assays for the diagnosis of tuberculosis (TB) can give indeterminate results. The prevalence of indeterminate test results (ITRs) among T-SPOT.TB tests was assessed. A retrospective analysis of samples processed in 2005 was performed. ITRs were assessed by age, sex, immunosuppression, distance to the laboratory and season. A subgroup of tests performed for specific indications (contact tracing, migrants with positive tuberculin skin test, TB suspects and immunosuppression) were analysed separately. Of a total of 1,429 tests, 49 (3.4%) were indeterminate. ITRs were significantly associated with old age (>75 versus 5-75 yrs; odds ratio (OR) 7.97, 95% confidence interval (CI) 3.968-15.438) and the season during which samples were transported (autumn and winter versus spring and summer; OR 3.47, 95% CI 1.753-7.514). The incidence of ITR was 302 (2.0%) among TB contacts, 75 (1.6%) among immigrants, 156 (3.0%) in TB suspects and 32 (3.0%) among immunosuppressed patients. Sex, young age and distance to the laboratory were not associated with the rate of ITR. Of the 13 tests with ITR that were repeated, 10 gave a clear positive or negative result. Indeterminate test results with T-SPOT.TB under routine conditions were infrequent and more common in individuals aged >75 yrs than in children and younger adults. The incidence of indeterminate test results was low and similar among healthy tuberculosis contacts, immigrants with a positive tuberculin skin test, tuberculosis suspects and the immunosuppressed. The conditions of transportation may influence the incidence of indeterminate test results.
Mots-clé
Adolescent , Adult , Age Factors , Aged , Aged,80 and over , Ambulatory Care , analysis , blood , Child , Child,Preschool , Contact Tracing , diagnosis , Female , Humans , Incidence , Infant , Interferon-gamma , Male , Mass Screening , metabolism , methods , microbiology , Middle Aged , Odds Ratio , Patients , Prevalence , Reagent Kits,Diagnostic , Retrospective Studies , Sensitivity and Specificity , Sex , Skin , Switzerland , T-Lymphocytes , Transportation , Tuberculosis
Pubmed
Web of science
Création de la notice
29/01/2009 22:13
Dernière modification de la notice
20/08/2019 16:07
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