TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_E3A6480CBEC0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
Périodique
Journal of Immunology
Auteur(s)
Perrot I., Deauvieau F., Massacrier C., Hughes N., Garrone P., Durand I., Demaria O., Viaud N., Gauthier L., Blery M., Bonnefoy-Berard N., Morel Y., Tschopp J., Alexopoulou L., Trinchieri G., Paturel C., Caux C.
ISSN
1550-6606 ([electronic])
0022-1767 ([linking])
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
185
Numéro
4
Pages
2080-2088
Langue
anglais
Résumé
Cross-talk between NK cells and dendritic cells (DCs) is critical for the potent therapeutic response to dsRNA, but the receptors involved remained controversial. We show in this paper that two dsRNAs, polyadenylic-polyuridylic acid and polyinosinic-polycytidylic acid [poly(I:C)], similarly engaged human TLR3, whereas only poly(I:C) triggered human RIG-I and MDA5. Both dsRNA enhanced NK cell activation within PBMCs but only poly(I:C) induced IFN-gamma. Although myeloid DCs (mDCs) were required for NK cell activation, induction of cytolytic potential and IFN-gamma production did not require contact with mDCs but was dependent on type I IFN and IL-12, respectively. Poly(I:C) but not polyadenylic-polyuridylic acid synergized with mDC-derived IL-12 for IFN-gamma production by acting directly on NK cells. Finally, the requirement of both TLR3 and Rig-like receptor (RLR) on mDCs and RLRs but not TLR3 on NK cells for IFN-gamma production was demonstrated using TLR3- and Cardif-deficient mice and human RIG-I-specific activator. Thus, we report the requirement of cotriggering TLR3 and RLR on mDCs and RLRs on NK cells for a pathogen product to induce potent innate cell activation.
Mots-clé
Adaptor Proteins, Signal Transducing/genetics, Adaptor Proteins, Signal Transducing/metabolism, Animals, Cell Line, Cells, Cultured, DEAD-box RNA Helicases/genetics, DEAD-box RNA Helicases/metabolism, Dendritic Cells/cytology, Dendritic Cells/drug effects, Dose-Response Relationship, Drug, Humans, Interferon-gamma/metabolism, Killer Cells, Natural/cytology, Killer Cells, Natural/drug effects, Lymphocyte Activation/drug effects, Mice, Mice, Inbred C57BL, Mice, Knockout, Myeloid Cells/cytology, Myeloid Cells/drug effects, Poly A-U/pharmacology, Poly I-C/pharmacology, RNA, Double-Stranded/pharmacology, Toll-Like Receptor 3/genetics, Toll-Like Receptor 3/metabolism, Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/09/2010 16:19
Dernière modification de la notice
20/08/2019 17:07
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