Identification of the minimal promoter region of the mouse NKX5-3, a transcription factor implicated in eye development.

Détails

ID Serval
serval:BIB_E3711EF52364
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of the minimal promoter region of the mouse NKX5-3, a transcription factor implicated in eye development.
Périodique
Gene
Auteur⸱e⸱s
Nichini O., Schorderet D.F.
ISSN
0378-1119
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
411
Numéro
1-2
Pages
10-8
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
Early ocular development is controlled by a complex network of transcription factors, cell cycle regulators, and diffusible signalling molecules. Together, these molecules regulate cell proliferation and apoptosis, and specify retinal fate. NKX5-3 is a homeobox transcription factor implicated in eye development. The analysis of the 5'-flanking region of the mouse Nkx5-3 gene revealed a predicted TATA-less promoter sequence between -416 and -166 of the translation start site. To functionally characterise Nkx5-3 promoter activity, serial deletions of the promoter sequence were introduced in pGL-3 basic vector and promoter activity of these 5'- and 3'-deleted constructions was tested in HeLa and CHO cells. Transactivation assays identified a region between -350 and -296 exhibiting promoter-like activity. Combined analysis by deletions and point mutations showed that this sequence, containing multiple Sp1 binding sites was necessary to promote transcriptional activity. Binding of Sp1 to this region was confirmed by electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation, using an antibody specific for Sp1. Altogether, these results demonstrated that the immediate upstream region of Nkx5-3 gene possessed a strong intrinsic promoter activity in vitro, suggesting a potential role in Nkx5-3 transcription in vivo.
Mots-clé
5' Flanking Region, Animals, Base Sequence, Eye/embryology, Mice, Molecular Sequence Data, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid, Sp1 Transcription Factor/metabolism, Transcription Factors/genetics, Transcriptional Activation
Pubmed
Web of science
Création de la notice
05/10/2009 13:40
Dernière modification de la notice
20/08/2019 16:07
Données d'usage