Unidirectional and oscillatory shear stress differentially modulate NOS III gene expression

Détails

ID Serval
serval:BIB_E356F7F69152
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Unidirectional and oscillatory shear stress differentially modulate NOS III gene expression
Périodique
Nitric Oxide
Auteur(s)
Silacci  P., Formentin  K., Bouzourene  K., Daniel  F., Brunner  H. R., Hayoz  D.
ISSN
1089-8603 (Print)
Statut éditorial
Publié
Date de publication
02/2000
Volume
4
Numéro
1
Pages
47-56
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
Atherosclerotic plaques preferentially develop in regions exposed to a low mean shear stress and cyclic reversal of flow direction (oscillatory flow). This contrasts with plaque-free zones where endothelial cells are exposed to unidirectional flow. Previous works from our laboratory using a unique experimental flow system demonstrated the existence of a differential regulation of endothelial nitric oxide synthase (NOS III) gene expression by unidirectional and oscillatory flow patterns. We further studied the possible mechanisms responsible for selective unresponsiveness of NOS III gene regulation to oscillatory flow. The results obtained demonstrate that (i) induction of the activity of the 1600-base-pair NOS III gene promoter by unidirectional and oscillatory shear stress is modulated by similar mechanisms that involve NF-kappaB activation, but do not involve Ras-dependent MAP kinase activation, and (ii) the lack of induction of NOS III gene regulation by oscillatory shear stress can be attributed to the activation of a yet unidentified negative cis-acting element present in the NOS III gene.
Mots-clé
Base Sequence Cells, Cultured Enzyme Activation Gene Expression Regulation, Enzymologic/*physiology Mitogen-Activated Protein Kinases/metabolism NF-kappa B/physiology Nitric Oxide Synthase/*genetics Nitric Oxide Synthase Type III Promoter Regions (Genetics) Stress, Mechanical
Pubmed
Web of science
Création de la notice
17/01/2008 17:38
Dernière modification de la notice
20/08/2019 17:07
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