Screening with a shell vial assay for antiviral activity against cytomegalovirus
Détails
ID Serval
serval:BIB_E353C15E4010
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Screening with a shell vial assay for antiviral activity against cytomegalovirus
Périodique
Diagnostic Microbiology and Infectious Disease
ISSN
0732-8893 (Print)
Statut éditorial
Publié
Date de publication
02/1989
Volume
12
Numéro
1
Pages
5-8
Notes
Journal Article --- Old month value: Jan-Feb
Résumé
An assay based on the shell vial cell culture (SVA) was devised to assess differences in fluorescence patterns of 8 cytomegalovirus (CMV) strains incubated in the presence or absence of three antiviral substances. The drug concentrations selected for in vitro testing were in the range of peak serum levels achievable in humans (ganciclovir 20 microM, acyclovir 80 microM, and phosphonoformic acid 300 microM). Four days after inoculation of the shell vials, the cell monolayer was fixed, stained with a mixture of monoclonal antibodies to early and late antigens of CMV, and reacted with antimouse fluorescein-labeled globulin. Twenty microM of ganciclovir in the media inhibited the formation of plaque-like foci and specific cytoplasmic fluorescence of laboratory strain AD169 and four clinical isolates. Two documented resistant strains exhibited partial inhibition at 20 microM, and a clinical isolate exhibited incomplete inhibition in three or four occasions. None of the strains was fully inhibited by 80 microM of acyclovir; however, different degrees of partial inhibition were observed. All isolates exhibited high-grade inhibition by 300 microM of phosphonoformic acid. The SVA can be used as a simple screening test for anti-CMV substances and as a screening of CMV resistance to ganciclovir prior to quantitative testing by the more complex plaque reduction assay.
Mots-clé
Acyclovir/*analogs & derivatives/*pharmacology
Antiviral Agents/*pharmacology
Cell Line
Cytomegalovirus/*drug effects
Fluorescent Antibody Technique
Foscarnet
Ganciclovir
Humans
Organophosphorus Compounds/*pharmacology
Phosphonoacetic Acid/analogs & derivatives/*pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 15:45
Dernière modification de la notice
20/08/2019 17:07