The N-terminal domain of Plasmodium falciparum circumsporozoite protein represents a target of protective immunity.

Détails

ID Serval
serval:BIB_E31ACCD055B5
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
The N-terminal domain of Plasmodium falciparum circumsporozoite protein represents a target of protective immunity.
Périodique
Vaccine
Auteur⸱e⸱s
Bongfen S.E., Ntsama P.M., Offner S., Smith T., Felger I., Tanner M., Alonso P., Nebie I., Romero J.F., Silvie O., Torgler R., Corradin G.
ISSN
0264-410X
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
27
Numéro
2
Pages
328-335
Langue
anglais
Résumé
The N-terminal domain of the circumsporozoite protein (CSP) has been largely neglected in the search for a malaria vaccine in spite of being a target of inhibitory antibodies and protective T cell responses in mice. Thus, in order to develop this region as a vaccine candidate to be eventually associated with other candidates and, in particular, with the very advanced C-terminal counterpart, synthetic constructs representing N- and C-terminal regions of Plasmodium falciparum and Plasmodium berghei CSP were administered as single or combined formulations in mice. We show that the antisera generated against the combinations inhibit sporozoite invasion of hepatocytes in vitro better than antisera against single peptides. Furthermore, two different P. falciparum CSP N-terminal constructs (PfCS22-110 and PfCS65-110) were recognized by serum samples from people living in malaria-endemic regions. Importantly, recognition of the short N-terminal peptide (PfCS65-110) by sera from children living in a malaria-endemic region was associated with protection from disease. Taken together, these results underline the potential of using such fragments as malaria vaccine candidates.
Mots-clé
Amino Acid Sequence, Animals, Antibodies, Protozoan/blood, Antibodies, Protozoan/immunology, Child, Preschool, Female, Hepatocytes/parasitology, Humans, Malaria/immunology, Malaria/parasitology, Malaria Vaccines, Malaria, Falciparum/diagnosis, Malaria, Falciparum/immunology, Mice, Molecular Sequence Data, Peptide Fragments/chemical synthesis, Peptide Fragments/chemistry, Plasmodium berghei/immunology, Plasmodium falciparum/growth & development, Plasmodium falciparum/immunology, Protozoan Proteins/chemistry, Protozoan Proteins/immunology
Pubmed
Web of science
Création de la notice
29/10/2009 16:18
Dernière modification de la notice
20/08/2019 16:06
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