Electron microscopic visualization of protein-DNA interactions at the estrogen responsive element and in the first intron of the Xenopus laevis vitellogenin gene.

Détails

ID Serval
serval:BIB_E2C64E755C59
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Electron microscopic visualization of protein-DNA interactions at the estrogen responsive element and in the first intron of the Xenopus laevis vitellogenin gene.
Périodique
EMBO Journal
Auteur⸱e⸱s
ten Heggeler-Bordier B., Hipskind R., Seiler-Tuyns A., Martinez E., Corthésy B., Wahli W.
ISSN
0261-4189[print], 0261-4189[linking]
Statut éditorial
Publié
Date de publication
06/1987
Volume
6
Numéro
6
Pages
1715-1720
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Stable protein-DNA complexes can be assembled in vitro at the 5' end of Xenopus laevis vitellogenin genes using extracts of nuclei from estrogen-induced frog liver and visualized by electron microscopy. Complexes at the three following sites can be identified on the gene B2: the transcription initiation site, the estrogen responsive element (ERE) and in the first intron. The complex at the transcription initiation site is stabilized by dinucleotides and thus represents a ternary transcription complex. The formation of the complexes at the two other sites is enhanced by estrogen and is reduced by tamoxifen, an antagonist of estrogen, while this latter effect is reversed by adding an excess of hormone. No sequence homology is apparent between the site containing the ERE and the binding site in intron I and functional tests in MCF-7 cells suggest that these two sites are not equivalent. Finally, we made use of previously characterized deletion mutants of the 5' flanking region of the gene B1, a close relative of the gene B2, to demonstrate that the 13-bp palindromic core element of the ERE is involved in the formation of the complexes observed upstream of the transcription initiation site.
Mots-clé
Animals, Cell Nucleus/drug effects, Cell Nucleus/metabolism, DNA/metabolism, DNA/ultrastructure, Estradiol/pharmacology, Female, Genes/drug effects, Introns, Liver/drug effects, Liver/metabolism, Microscopy, Electron, Plasmids, Promoter Regions, Genetic, Proteins/metabolism, Tamoxifen/pharmacology, Transcription, Genetic, Vitellogenins/genetics, Xenopus laevis
Pubmed
Web of science
Création de la notice
25/01/2008 15:53
Dernière modification de la notice
20/08/2019 17:06
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