Role of the cystic fibrosis transmembrane conductance regulator in internalization of Pseudomonas aeruginosa by polarized respiratory epithelial cells

Détails

ID Serval
serval:BIB_E1BEA06B8F3C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Role of the cystic fibrosis transmembrane conductance regulator in internalization of Pseudomonas aeruginosa by polarized respiratory epithelial cells
Périodique
Cell Microbiol
Auteur(s)
Darling K. E., Dewar A., Evans T. J.
ISSN
1462-5814 (Print)
ISSN-L
1462-5814
Statut éditorial
Publié
Date de publication
06/2004
Volume
6
Numéro
6
Pages
521-33
Langue
anglais
Notes
Darling, Katharine E A
Dewar, Ann
Evans, Thomas J
eng
Research Support, Non-U.S. Gov't
England
Cell Microbiol. 2004 Jun;6(6):521-33. doi: 10.1111/j.1462-5822.2004.00380.x.
Résumé
Pseudomonas aeruginosa is an important human pathogen, producing lung infection in individuals with cystic fibrosis (CF), patients who are ventilated and those who are neutropenic. The respiratory epithelium provides the initial barrier to infection. Pseudomonas aeruginosa can enter epithelial cells, although the mechanism of entry and the role of intracellular organisms in its life cycle are unclear. We devised a model of infection of polarized human respiratory epithelial cells with P. aeruginosa and investigated the role of the cystic fibrosis transmembrane conductance regulator (CFTR) in adherence, uptake and IL-8 production by human respiratory epithelial cells. We found that a number of P. aeruginosa strains could invade and replicate within cells derived from a patient with CF. Intracellular bacteria did not produce host cell cytotoxicity over a period of 24 h. When these cells were transfected with wild-type CFTR, uptake of bacteria was significantly reduced and release of IL-8 following infection enhanced. We propose that internalized P. aeruginosa may play an important role in the pathogenesis of infection and that, by allowing greater internalization into epithelial cells, mutant CFTR results in an increased susceptibility of bronchial infection with this microbe.
Mots-clé
Apoptosis, Bacterial Adhesion, Cell Line, Cell Polarity, Colony Count, Microbial, Cystic Fibrosis/microbiology, Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism, Cytoplasm/microbiology, Epithelial Cells/*microbiology/ultrastructure, Gene Deletion, Humans, Interleukin-8/analysis/biosynthesis, Microscopy, Confocal, Microscopy, Electron, Mutation, Pseudomonas aeruginosa/growth & development/*physiology, Respiratory Mucosa/*microbiology/ultrastructure
Pubmed
Création de la notice
07/04/2020 14:20
Dernière modification de la notice
08/04/2020 5:26
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