Nuclear migration of NF-kappa B correlates with TNF-alpha mRNA accumulation
Détails
ID Serval
serval:BIB_E18A98586AEF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Nuclear migration of NF-kappa B correlates with TNF-alpha mRNA accumulation
Périodique
Journal of Inflammation
ISSN
1078-7852 (Print)
Statut éditorial
Publié
Date de publication
1995
Volume
45
Numéro
1
Pages
64-71
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Résumé
Previous work on the transcriptional regulation of the mouse TNF-alpha promoter had indicated a major role for the NF-kappa B transcription factor in the induction of the gene by endotoxin. However, similar studies using the human promoter failed to establish a role for this factor. We measured the nuclear migration of NF-kappa B and the accumulation of TNF-alpha mRNA in murine T lymphoblasts and bone marrow-derived macrophages (BMDM) under different activation conditions, seeking to establish a correlation. Activation of NF-kappa B and accumulation of TNF-alpha mRNA correlated semiquantitatively under the following conditions: (1) inhibition of NF-kappa B by dithiocarbamates; (2) induction of TNF synthesis by taxol; (3) partial induction of TNF-alpha mRNA by various inducers in macrophages from lpsd mice; and (4) inhibition of NF-kappa B activation by a protease inhibitor. However, inhibition of TNF-alpha mRNA accumulation by cAMP inducers had no effect on NF-kappa B induction. We conclude that NF-kappa B translocation is necessary, but not sufficient for the transcriptional induction of the TNF-alpha gene by LPS in macrophages or by phorbol ester and ionomycin in T lymphocytes.
Mots-clé
Animals
Base Sequence
Biological Transport
Bone Marrow Cells
Cell Nucleus/*metabolism
Cells, Cultured
Cyclic AMP/metabolism
Free Radical Scavengers/pharmacology
Macrophages/metabolism/ultrastructure
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Molecular Sequence Data
NF-kappa B/*metabolism
Nocodazole/pharmacology
Paclitaxel/pharmacology
RNA, Messenger/*metabolism
Serine Proteinase Inhibitors/pharmacology
T-Lymphocytes/metabolism/ultrastructure
Tosylphenylalanyl Chloromethyl Ketone/pharmacology
Tumor Necrosis Factor-alpha/*genetics
Pubmed
Web of science
Création de la notice
24/01/2008 16:39
Dernière modification de la notice
20/08/2019 17:05